Today’s rapid development of powerful tools for gene expression analysis provides unprecedented resources for elucidating complex molecular events. The objective of this work has been to apply, combine and evaluate tools for analysis of differential gene expression using atherosclerosis as a model system. First, an optimised solid-phase protocol for representational difference analysis (RDA) wa...

Representational difference analysis (RDA) is a powerful technique for cloning the differences between genomes, and has recently been adapted for cloning differentially expressed genes. RDA, like other PCR-based differential screening methods, is prone to the production of false positives. We have identified a major source of false positives in RDA of cDNA and have introduced improvements which...

Resource Description & Access (RDA) is intended to provide a flexible and extensible framework that can accommodate all types of content and media within rapidly evolving digital environments while also maintaining compatibility with the Anglo-American Cataloguing Rules, 2nd edition (AACR2). The cataloging community is grappling with practical issues in navigating the transition from AACR2 to R...

BACKGROUND The establishment of staff canteens is expected to improve the nutritional situation of Cambodian garment workers. The objective of this study is to assess the nutritive value of low-price model lunch sets provided at a garment factory in Phnom Penh, Cambodia. METHODS Exemplary lunch sets were served to female workers through a temporary canteen at a garment factory in Phnom Penh. ...

The management of vocabularies in the evolving linked data environment requires different tools and processes than libraries and other memory institutions have used in the past. The RDA Resource Description and Access standard has taken the lead in building tools and providing services as part of its RDA Registry development. The evolution of the current RDA Registry and the Open Metadata Regis...

OBJECTIVE The aim of this study was to validate the EAR cut-point method for assessing the prevalence of nutrient inadequacy at the population level. DESIGN AND SUBJECTS Different methods for estimating the prevalence of inadequate intake were compared: the cut-off point method, with cut-off points at the Recommended Dietary Allowance (RDA), 0.66 RDA, 0.50 RDA and the Estimated Average Requir...

The rotating discs argument (RDA) against perdurantism has been mostly discussed by metaphysicians, though the argument of course appeals to ideas from classical mechanics, especially about rotation. In contrast, I assess the RDA from the perspective of the philosophy of physics. I argue for three main conclusions. The first conclusion is that the RDA can be formulated more strongly than is usu...

Lysosomes can degrade various biological macromolecules, including nucleic acids, proteins and lipids. Recently, we identified novel nucleic acid-degradation systems termed RNautophagy/DNautophagy (abbreviated as RDA), in which RNA and DNA are directly taken up by lysosomes in an ATP-dependent manner and degraded. We also found that a lysosomal membrane protein, LAMP2C, the cytoplasmic region o...