Simple, rapid, and extractive spectrophotometric methods were developed for the determination of gatifloxacin (GT) in bulk and pharmaceutical dosage form. These methods are based on the formation of yellow ion-pair complexes between the basic nitrogen of the drug and three sulphonphthalein acid dyes, namely; bromocresol green (BCG), bromocresol purple (BCP), bromophenol blue (BPB) and bromothym...

BACKGROUND Serum/plasma albumin is an important predictor of future mortality/morbidity in haemodialysis (HD) patients and has been proposed as an important audit measure. Different methods of albumin assay give different results and the bias between methods may be greater in renal failure patients. METHODS Albumin concentration in plasma was measured by three methods, two dye-binding methods...

Calculated globulin (total protein - albumin) is usually tested as part of a liver function test profile in both primary and secondary care and determines the serum globulin concentration, of which immunoglobulins are a major component. The main use hitherto of calculated globulin is to detect paraproteins when the level is high. This study investigated the potential to use low levels of calcul...

Three sensitive, selective, rapid and easily reproducible spectrophotometric methods (A-C) have been developed for the determination of propranolol hydrochloride (PPH) as a hydrochloride free base propranolol (PPL) in bulk sample and in its dosage forms. These methods are based on ion-pair formation between propranolol as a hydrochloride and free base and three acidic (sulphonphthalein) dyes; n...

A direct, extraction-free spectrophotometric method has been developed for the determination of acebutolol hydrochloride (ABH) in pharmaceutical preparations. The method is based on ionpair complex formation between the drug and two acidic dyes (sulphonaphthalein) namely bromocresol green (BCG) and bromothymol blue (BTB). Conformity to Beer’s law enabled the assay of the drug in the range of 0....

The removal of model pollutants bromocresol green (BG) and phenol from water is demonstrated via two liquid-liquid extraction methods. Both methods exploit selective interactions established by the pollutant molecule with a surfactant, oil, or alcohol, and are variants of the more general Winsor systems where the phases are in contact along an extremely large interfacial area. In the first meth...

A sensitive and rapid extractive spectrophotometer method has been developed for the assay of carvedilol in pharmaceutical formulations. The method is based on the formation of a chloroform soluble ion-pair complex between carvedilol and bromocresol green in an acidic medium. The complex shows absorption maximum at 415 nm and the system obeys Beer’s law in the concentration range of 5-25 μg/ml....

The study is to evaluate the effect of age and sex on serological indices of Japanese quail (Coturnix coturnix japonica) reared in derived savanna area of Nigeria. A total of 200 quails comprising 100 each of male and female were selected for this study. Fifteen each of the males and females quails were randomly selected at each week of 6, 7, 8, 9, 10 and 11 respectively. Data were obtained on ...

The methods for determination of chitosan content recommended in the Chinese Pharmacopoeia and the European Pharmacopoeia are not applicable for evaluation of the extent of deacetylation (deacetylation degree, DD) in chitooligosaccharides (COS). This study explores two different methods for assessment of DD in COS having relatively high and low molecular weights: an acid-base titration with bro...

In most clinical chemistry laboratories, mechanized procedures are essential for measurement of serum total protein and albumin. The biuret reaction is well established as the most practical approach to mechanized total protein measurements (1-4); albumin has posed a more difficult problem, with several procedures being proposed, some of which have involved salt fractionation (1-4), electrophor...