The dopamine (DA) transporter (DAT) facilitates high-affinity presynaptic DA reuptake that temporally and spatially constrains DA neurotransmission. Aberrant DAT function is implicated in attention-deficit/hyperactivity disorder and autism spectrum disorder. DAT is a major psychostimulant target, and psychostimulant reward strictly requires binding to DAT. DAT function is acutely modulated by d...

To examine the oligomeric state and trafficking of the dopamine transporter (DAT) in different compartments of living cells, human DAT was fused to yellow (YFP) or cyan fluorescent protein (CFP). YFP-DAT and CFP-DAT were transiently and stably expressed in porcine aortic endothelial (PAE) cells, human embryonic kidney (HEK) 293 cells, and an immortalized dopaminergic cell line 1RB3AN27. Fluores...

background: visceral leishmaniasis is systematic serous parasitic disease with pub­lic health importance. zoonotic form of visceral leishmaniasis is wide spread in mediterranean basin and south america regions. direct agglutination test (dat) is an accurate, reliable and non-expensive serological test for the diagnosis of visceral leishmaniasis in human and canines but the antigen preparation i...

Cocaine's ability to block the dopamine transporter (DAT) is crucial for its reinforcing effects. However the brain functional consequences of DAT blockade by cocaine are less clear since they are confounded by its concomitant blockade of norepinephrineand serotonin transporters. To separate the dopaminergic from the non-dopaminergic effects of cocaine on brain function we compared the regional...

An experiment was carried out to assess the effect of four nitrogen levels viz. T1 (full doze of urea i.e. 215 kg urea ha-1 at 15 DAT), T2 (full doze of urea at two equal splits, ½ at 15 DAT + ½ at 30 DAT), T3 (full doze of urea at two equal splits, ½ at 15 DAT + ½ at 55 DAT) and T4 (full doze of urea at three equal splits, 1/3 at 15 DAT + 1/3 at 30 DAT + 1/3 at 55 DAT) on morpho-physiological ...

Frequency of irrigation to the crops is the key factor for proper growth and economic yield. Therefore, in the current study growth of broccoli plants was investigated under different irrigation levels in pot culture condition. Five levels of irrigation schedules were applied including I0 [Non-irrigation control], I1 [Irrigation up to 15 days after transplanting (DAT)], I2 [Irrigation up to 30 ...

UNLABELLED The dopamine (DA) transporter (DAT) controls dopaminergic neurotransmission by removing extracellular DA. Although DA reuptake is proposed to be regulated by DAT traffic to and from the cell surface, the membrane trafficking system involved in the endocytic cycling of DAT in the intact mammalian brain has not been characterized. Hence, we performed immunolabeling and quantitative ana...

Dopaminergic signaling profoundly impacts rewarding behaviors, movement, and executive function. The presynaptic dopamine (DA) transporter (DAT) recaptures released DA, thereby limiting synaptic DA availability and maintaining dopaminergic tone. DAT constitutively internalizes and PKC activation rapidly accelerates DAT endocytosis, resulting in DAT surface loss. Longstanding evidence supports P...

The dopamine transporter (DAT) removes dopamine from the extracellular milieu and is potently inhibited by number of psychoactive drugs, including cocaine, amphetamines, and methylphenidate (Ritalin). Multiple lines of evidence demonstrate that protein kinase C (PKC) down-regulates dopamine transport, primarily by redistributing DAT from the plasma membrane to endosomal compartments, although t...