Background The extracellular matrix (ECM) can directly or indirectly influence on regulation of cell functions such as cell adhesion, migration, proliferation and differentiation. The cell derived ECM (CD-ECM) is a useful in vitro model for studying the comprehensive functions of CD-ECM because it maintains a native-like structure and composition. In this study, the CD-ECM is obtained and a tes...

OBJECTIVE Adipose tissue shows a high degree of plasticity, and adipocyte hyperplasia is an important mechanism for adipose tissue expansion. Different adipose depots respond differently to an increased demand for lipid storage. Orchestrating cellular expansion in vivo requires extracellular matrix (ECM) remodeling and a high degree of interaction between cells and ECM. METHODS We studied dec...

Filopodia have a key role in sensing both chemical and mechanical cues in surrounding extracellular matrix (ECM). However, quantitative understanding is still missing in the filopodial mechanosensing of local ECM stiffness, resulting from dynamic interactions between filopodia and the surrounding 3D ECM fibers. Here we present a method for characterizing the stiffness of ECM that is sensed by f...

background and objectives: biological scaffold resources composed of extracellular matrix (ecm) have been shown to make easy the practical remodeling of various tissues in both animal and human studies. the goal of current study was to make sheet form of ecm from sheep’s urinary bladder. methods: ecm was extracted from sheep’s urinary bladder according to standard method. scanning electron mic...

Extracellular matrix (ECM), prepared from chick embryo fibroblasts, contains fibronectin as the major structural protein along with collagen and other polypeptides as less abundant protein components. When Rous sarcoma virus-transformed chick embryo fibroblasts are cultured on the ECM in the presence of the tumor promoter tetradecanoyl phorbol acetate, the transformed cells lose their character...

The extracellular matrix (ECM) of the dermis is a critical structural component required for normal wound healing. The ECM, along with its key signaling components, provides the support structure necessary for fibroblasts, immune cells, and keratinocytes. When ECM activity is dysfunctional, the normal wound healing process is compromised, leading to slow, irregular repair, which may result in t...

introduction: extracellular matrix (ecm) components significantly influence the growth characteristics of cell, development of spontaneous contractile activity, andmorphologic differentiation. the present study evaluates the effect of ecm ongenotypic and physiologic characteristics of cardiomyocytes derived from embryonic stem (es) cells. material and methods: embryoid bodies derived from mouse...