Recombinant engineering using Red recombinase-based approaches offers efficient and rapid approaches to deletion and modification of genes. Here we describe a novel application of Red recombinant engineering that enables direct manipulation of chromosomal loci by electroporation with short synthetic DNA molecules. We demonstrate the use of this approach for the generation of scarless in-frame d...

We have developed a simple and highly efficient method to disrupt chromosomal genes in Escherichia coli in which PCR primers provide the homology to the targeted gene(s). In this procedure, recombination requires the phage lambda Red recombinase, which is synthesized under the control of an inducible promoter on an easily curable, low copy number plasmid. To demonstrate the utility of this appr...

The circumventricular organs (CVOs) lack a well-formed blood-brain barrier and produce superoxide in response to angiotensin II and other hypertensive stimuli. This increase in central superoxide has been implicated in the regulation of blood pressure. The extracellular superoxide dismutase (SOD3) is highly expressed in cells associated with CVOs and particularly with tanycytes lining this regi...

This paper presents both, the design and the development of a monitoring and control system via web for a closed microalgae crop and the results that were gotten using the strain Haematococcus pluvialis. The research was done at Sabana University (Colombia) and it aims to quantify the kinetic growth associated to the increment of biomass and the development of red pigment inside the cells when ...

shigella species (spp.) are gram-negative bacteria that are responsible for shigellosis. although it can be controlled via antibiotics, increasing number of antibiotic resistant isolates of shigella have been reported. therefore, other strategies such as production of specific vaccine against this organism could be a suitable therapeutic approach. attenuated live vaccines produced by gene delet...

We retrieved the mass-specific scattering coefficient b*sm(λ) = 0.60·(λ/650) of the inhomogeneous and optically complex water column of eastern Lake Constance in May 2012. In-situ measured and modelled remote-sensing reflectance Rrs(λ) were matched via a parameter search procedure using genetic algorithms. The optical modelling consisted of solving the azimuthally-averaged Radiative Transfer Eq...

First cohomology groups of finite groups with nontrivial irreducible coefficients have been useful in several geometric and arithmetic contexts, including Wiles’s famous paper (1995). Internal to group theory, 1-cohomology plays a role in the general theory of maximal subgroups of finite groups, as developed by Aschbacher and Scott (1985).One can pass to the case where the group acts faithfully...

Evidence for the recognition of a secondary structure of carrageenan chains present in red algae tissues. Abstract We report the isolation, for the first time by phage display, of a scFv recombinant antibody called B3 directed against carrageenans, the major sulphated polysaccharides of red seaweeds. Immunoassays were used to characterize the binding of B3 antibodies towards the three main carr...