Recombination of λ red gam phage in recD mutants is unaffected by inactivation of RecJ exonuclease. Since nucleases play redundant roles in E. coli, we inactivated several exonucleases in a recD mutant and discovered that 5’-3’ exonuclease activity of RecJ and exonuclease VII is essential for λ recombination, whereas exonucleases of 3’-5’ polarity are dispensable. The implications of the presen...

Phytochromes are red/far-red light photoreceptors that convert the information contained in external light into biological signals. The decoding process starts with the perception of red light, which occurs through photoisomerization of a chromophore located within the phytochrome, leading to structural changes that include the disruption of intramolecular interactions between the N- and C-term...

Shigella species (spp.) are gram-negative bacteria that are responsible for shigellosis. Although it can be controlled via antibiotics, increasing number of antibiotic resistant isolates of Shigella have been reported. Therefore, other strategies such as production of specific vaccine against this organism could be a suitable therapeutic approach. Attenuated live vaccines produced by gene delet...

Suppose λ,x,ζ traverse the ordered sets Λ, X and Z, respectively and consider the functions f(λ,x,ζ) and g(λ,ζ) satisfying the following conditions, (a) f(λ,x,ζ) > 0 and f is TP2 in each pairs of variables when the third variable is held fixed and (b) g(λ,ζ) is TP2. Then the function h(λ,x) =∫Z f(λ,x,ζ)g(λ,ζ)dµ(ζ), defined on Λ×X is TP2 in (λ,x). The aim of this note is to use a new stochast...

We explore the macro-financial consequences of disruption a supply chain in an agent based framework characterized by two networks, credit network connecting banks and firms production upstream down-stream firms. consider scenarios. In first one, because lockdown all are forced to cut production. This generates sizable down-turn during due indirect effects shock (network financial accelerator)....

Caged rhodamine dyes (Rhodamines NN) of five basic colors were synthesized and used as "hidden" markers in subdiffractional and conventional light microscopy. These masked fluorophores with a 2-diazo-1-indanone group can be irreversibly photoactivated, either by irradiation with UV- or violet light (one-photon process), or by exposure to intense red light (λ∼750 nm; two-photon mode). All dyes p...

Chase-escape is a competitive growth process in which red particles spread to adjacent empty sites according rate-λ Poisson while being chased and consumed by blue rate-1 process. Given growing sequence of finite graphs, the critical rate λc largest value λ for fails reach positive fraction vertices with high probability. We provide conjecturally sharp lower bound an implicit upper on supercrit...

Successive structural changes of bacteriophage λ upon heating were characterized with quantitative experimental methods. In the commonly used Tris-Mg buffer, differential scanning calorimetry measurements first established that the protein capsid of λ phage melts at 87 °C and its genomic DNA melts at 91 °C. Interestingly, prior to the capsid melting, λDNA was found to escape out of the capsid a...

The phage lambda-derived Red recombination system is a powerful tool for making targeted genetic changes in Escherichia coli, providing a simple and versatile method for generating insertion, deletion, and point mutations on chromosomal, plasmid, or BAC targets. However, despite the common use of this system, the detailed mechanism by which lambda Red mediates double-stranded DNA recombination ...