objective(s): iran is considered as one of the high-prevalence areas for β-thalassemia with a rate of about 10% carrier frequency. molecular diagnosis of the disease is performed both by direct sequencing and indirectly by the use of polymorphic markers present in the beta globin gene cluster. however, to date there is no reliable information on the application of the markers in the iranian pop...

objective(s): β-thalassemia is one of the most common genetic disorders in the world. as one of the promising treatment strategies, fetal hemoglobin (hb f) can be induced. the present study was an attempt to reactivate the γ-globin gene by introducing a gene construct containing klf1 binding sites to the k562 cell line. materials and methods: a plasmid containing a 192 bp sequence with two repe...

introduction: β-globin gene cluster haplotypes are useful in diagnosis of particular molecular defects in β-thalassemia, prenatal diagnosis of β-thalassemia, and elucidating population affinities. methods: β-globin gene cluster haplotypes were studied in 150 β-thalassemia minor and 52 healthy in-dividuals from the fars province of iran. dna was extracted from leukocytes of whole blood by phe-no...

In order to better understand the chiral recognition mechanisms of positively charged cyclodextrin (CD) derivatives, synthesis, pKa determination by 1H nuclear magnetic resonance (NMR)-pH titration and a comparative capillary electrophoretic (CE) study were performed with two series mono-substituted cationic single isomer CDs. The first selectors mono-(6-N-pyrrolidine-6-deoxy)-β-CD (PYR-β-CD), ...

alzheimer’s disease (ad) is a neurodegenerative disorder and the most common form of dementia. almost 47 million people suffer from dementia worldwide. ad accounts for approximately 60%–80% of all dementia cases. three major pathologies characterize the disease: senile plaques, neurofibrillary tangles and inflammation. we review the literature on events contributing to the inflammation. those i...

background: β-thalassemia as a hereditary disease is defined as defective synthesis of   β-globin chains, resulting in erythropoiesis abnormalities and severe anemia. different studies have shown that cytokines and cytokine gene polymorphisms play a major role in the pathogenesis of   β-thalassemia. single nucleotide polymorphisms (snps) within the promoter region or other regulatory sequences ...

Background and objectives: Plumeria rubra L. (Apocynaceae) is a deciduous shrub or small tree cultivated in subtropical and tropical countries.  Its bark is prescribed to treat amoebic dysentery,   dropsy, jaundice, scabies, gonorrhea, venereal affections and wounds. In the present research, we aimed to isolate and identify the chemical constituents of the bark of ...