The metabolism of aflatoxin B, has been investigated in male rhesus monkeys given i.p. injections of a mixture of ring-labeled aflatoxin B!-'4C and nonradioactive toxin, at a total dose of 0.4 mg/kg. Thirty-five % of the administered radioactivity was excreted in urine within 96 hr. The excretion rate reached a maximum 1 hr after dosing and then fell rapidly, but urine excreted after 96 hr stil...

Human interferon ß(IFN-/Î) stimulated the synthesis of prostaglandin E (PGE) and prostaglandin F2o in IFN-sensitive RSa and GM258 cell lines, but not in IFN-resistant HEC-1 cells. IFN-/S at a concentration of 1000 units/ml elicited 2to 3-fold increases in PGE production in these cell lines. In the presence of exogenous arachidonic acid (1 jug/ml), IFN-pretreated cells produced 5-fold more PGE...

After i.v. administration of d,l-, /-5-formyltetrahydrofolate (d,lCHO-THF) CHO-THF was rapidly cleared from the plasma by conversion to 5-methyltetrahydrofolate (5-CH3-THF) and urinary excretion, whereas d-CHO-THF, which was not metabolized and was slowly excreted in the urine, persisted in plasma at concen trations greatly exceeding those of /-CHO-THF and 5-CH3-THF. The plasma half-life (ß) o...

Heparanase is an endo-ß-o-glucuronidase, the enzymatic targets of which are the glycosaminoglycan chains of heparan sulfate proteoglycans. Elevated levels of heparanase are associated with the metastatic potential of melanoma cells. Treatment of murine and human melanoma cells with the prototypic neurotrophin nerve growth factor (NGF) increases the production of heparanase by melanoma cells. W...

The association between high intake of fruit and vegetables and low incidence of certain cancers is well established. Dietary antioxidants pres ent in these foods are thought to decrease free radical attack on DNA and hence to protect against mutations that cause cancer, but this causal mechanism remains conjectural. We have adopted a molecular epidemi olÃ3gica!approach to this question, based ...

dimethylaminoazobenzene or Thioacetamide. Larry D. Smith and Donald E. Kizer. 1348 Hormonal Effects on Thymidine Kinase Ac tivity in Normal Rat Adrenal and in Hormonedependent Adrenal Carcinoma. M. R. Garland, Thérà ̈se Ng, and J. F. Richards. 1355 Erythropoietic Effect of Plasma from Patients with Advanced Cancer. Dincer Firat and Jose Banzon. 1360 Metabolic Studies on Mammary Tumor MTW9 follo...

GuliTiiii-3. an endogenous ß-galactoside-binding lectin. is present on colon cancer cells and may play a role in metastasis. Galcctin-3 hinds poly-.V-acetyllactosamine structures on glycoproteins. but its natural li gands remain to be fully defined. Galectin-3 bound to purified native and desialylated colon cancer mucin in a concentration-dependent manner, which »ascompletely inhibited by 0.1...

The levels of NAI)(P)H:(quinone-acceptor) oxidoreductase (EC.1.6. 99.2) (DT-diaphorase) mRNA and enzyme activity have been studied in paired human normal lung and non-small cell lung tumor samples from patients with a history of cigarette smoking. There were significantly higher levels of DT-diaphorase mRNA (1.2 kilobases) in lung tumor com pared to normal lung tissue of patients who had stoppe...

We developed a syngeneic mouse IgG2a monoclonal antibody (MAb) A9D41 directed against the Friend leukemia virus envelope gp70 antigen present on the cell surface membranes of virus producer 3C18 Friend leukemia cells (FLC). A9D41 showed a marked antitumor activity in DBA/2 mice given injections of gp70 positive 3C18 FLC, but it was ineffective in mice given injections of gp70 negative 745 FLC o...

Previous work in our laboratory has demonstrated that antisera to proteins found in the urine of bladder cancer patients can distinguish between urine samples from these patients and those from normal individuals when tested by a complementfixation assay. Antisera which were reactive with individual tumor-associated antigens were used to detect the antigens in the urine of bladder cancer patien...