The cellular slime mold, Dictyostelium mucoroides-7 (Dm7) exhibits clear dimorphism; macrocyst formation as a sexual process and sorocap formation as an asexual process. These two life cycles are regulated by two regulators, ethylene and cyclic AMP (cAMP). This is the first report demonstrating a novel function of ethylene at the cellular level. That is, ethylene induces a zygote formed by cell...

This paper presents the design and microfabrication of a vacuum-based cell holding device for single-cell immobilization and the use of the device in mouse zygote microinjection. The device contains many through-holes, constructed via two-sided glass wet etching and polydimethylsiloxane (PDMS)-glass bonding. Experimental results of mouse zygote immobilization and microinjection demonstrate that...

The Sox family plays essential roles as transcription factors in vertebrates; however, little is known about the Lutraria sieboldii. L. sieboldii are pleasant to eat with a short growth cycle and have become one of best bottom-seeded enrichment species Guang Xi. In this study, Sox2 (named LsSox2) Sox9 LsSox9) from were cloned, their expression patterns analyzed. length LsSox2 gene coding sequen...

To understand whether fusions of thylakoid membranes from the parental chloroplasts occurred during zygote formation in Chlamydomonas reinhardtii, we performed an ultrastructural analysis of the zygotes produced by crossing mutants lacking photosystem I or II protein complexes, in the absence of de novo chloroplast protein synthesis. Thylakoid membranes from each parent could be distinguished o...

The placenta contains several types of feto-maternal interfaces where zygote-derived cells interact with maternal cells or maternal blood for the promotion of fetal growth and viability. The genetic factors regulating the interactions between different cell types within feto-maternal interfaces and the relative contributions of the maternal and zygotic genomes are poorly understood. Genomic imp...