A rapid and sensitive spectrophotometric method is proposed for determination of Metronidazole. The method depends on the reduction of Metronidazole molecule with zinc dust and hydrochloric acid flowed by diazotation and coupling with 8-quinolinol to give colored chromogens easily measured spectrophotometrically which has λmax= 437 nm. The experimental conditions were optimized and berr’s law w...

Zinc is known to be an essential element for human nutrition and good health1). Milk is its sole source in infants, and growing children as well as mature adults consume varying amounts of milk, which provide them with much of the needed zinc. Investigators, using gel filtration chromatography2,3), have demonstrated that zinc in cow's milk can be associated with fractions of high molecular weig...

The structural integrity of the ubiquitous enzyme copper, zinc superoxide dismutase (SOD1) depends critically on the correct coordination of zinc and copper. We investigate here the roles of the stoichiometric zinc and copper ions in modulating the oxidative refolding of reduced, denatured bovine erythrocyte SOD1 at physiological pH and room temperature. Fluorescence experiment results showed t...

A postsynaptic membrane-associated protein of M(r) 43,000 (43-kD protein) is involved in clustering of the nicotinic acetylcholine receptor (AChR) at the neuromuscular junction. Previous studies have shown that recombinant mouse 43-kD protein forms membrane-associated clusters when expressed in Xenopus oocytes. Coexpression with the AChR results in colocalization of the receptor with the 43-kD ...

The nucleocapsid protein (NC) of HIV type 1 (HIV-1) is a nucleic acid chaperone that facilitates the rearrangement of nucleic acid secondary structure during reverse transcription. HIV-1 NC contains two CCHC-type zinc binding domains. Here, we use optical tweezers to stretch single lambda-DNA molecules through the helix-to-coil transition in the presence of wild-type and several mutant forms of...

Zinc plays a fundamental role in several critical cellular functions such as protein metabolism, gene expression, structural and functional integrity of biomembranes, and in metabolic processes [1]. Concentration of zinc in animals systems range from <10 M in cytoplasm to >10 M in some membrane vesicles [2]. Like all metal cations, zinc interacts electrostatically with anionic groups. In contra...

Zinc is an essential trace element for humans and plays a critical role both as a structural component of proteins and as a cofactor in about 300 enzymes. Zinc deficiency was, for example, reported to affect the immune response and the endocrine system and to induce and modify brain disorders. Besides hereditary zinc deficiency, zinc deficiency – at least in mild forms – is nowadays a very abun...

Halide, Pyridine Complexes, Zinc, X-Ray Data Zinc dichloride reacts with aminomethylpyridine (AMP) to the corresponding 1:1 adduct 1, whereas ZnBr2 forms an ionic 1:2 complex of the type [(AMP)2ZnBr]+ Br~ 2, which loses one neutral coligand upon heating in M e-C (0)0E t to yield nearly insoluble (AMP)ZnBr2 3. The condensation reaction of these compounds with acetone yields propylideneaminomethy...