Previously, we reported that coat protein (CP) of Wheat streak mosaic virus (WSMV) (genus Tritimovirus, family Potyviridae) tolerates deletion of amino acids 36 to 84 for efficient systemic infection of wheat. In this study, we demonstrated that WSMV mutants with deletion of CP amino acids 58 to 84 but not of 36 to 57 induced severe chlorotic streaks and spots, followed by acute chlorosis in wh...

Barley and Cereal yellow dwarf viruses (B/CYDVs), Wheat spindle streak mosaic (WSSMV), Soil-borne wheat mosaic virus (SBWMV) and Wheat streak mosaic virus (WSMV) constitute the most economically important group of wheat viruses. In this paper, a multiplex reverse transcription polymerase chain reaction (M-RT-PCR) method was developed for the simultaneous detection and discrimination of eight vi...

High plains disease (HPD) is of serious economic concern for wheat and corn production, but little is known about the virus-like causal agent. In the field, HPD is often associated with Wheat streak mosaic virus (WSMV) and both pathogens are transmitted by the same eriophyid wheat curl mite, Aceria tosichella Keifer. The objective of this study was to develop methods for establishing and mainta...

Wheat streak mosaic virus (WSMV) is a mite-vectored with substantial economic impact on wheat production. One of the effective sources resistance to WSMV, Wsm1, carried translocation from Thinopyrum intermedium. The original whole arm form this (T4DL·4JsS) was highly against WSMV but yield penalty in absence virus. Shorter recombinants Th. intermedium are now available. This study characterized...

McMechan, A. J., Tatineni, S., French, R., and Hein, G. L. 2014. Differential transmission of Triticum mosaic virus by wheat curl mite populations collected in the Great Plains. Plant Dis. 98:806-810. Wheat is an important food grain worldwide and the primary dryland crop in the western Great Plains. A complex of three wheat curl mite (WCM)-transmitted viruses (Wheat streak mosaic virus, High p...

Byamukama, E., Seifers, D. L., Hein, G. L., De Wolf, E., Tisserat, N. A., Langham, M. A. C., Osborne, L. E., Timmerman, A., and Wegulo, S. N. 2013. Occurrence and distribution of Triticum mosaic virus in the central Great Plains. Plant Dis. 97:21-29. Wheat curl mite (WCM)-transmitted viruses—namely, Wheat streak mosaic virus (WSMV), Triticum mosaic virus (TriMV), and the High Plains virus (HPV)...

Wheat streak mosaic (WSM), caused by Wheat streak mosaic virus (WSMV), is a devastating disease in wheat (Triticum aestivum L.) in the Great Plains of North America. Use of resistance is an effective and environmentally sound method to control the disease. In this study, six wheat genotypes were compared for their responses to WSMV infection under growth chamber conditions. The three resistant ...

Cereals play a significant role in the Ukrainian economy and agriculture. Viral diseases can cause serious reduction yields. Aim. The aim of work was to identify determine spread grain viruses main cereal-growing regions Ukraine period 2020–2021 using different methods virus detection. Methods. Enzyme-linked immunosorbent assay used commercial test systems by for wheat streak mosaic (WSMV), bar...

Previous workers in the lab created an infectious clone of Wheat Streak Mosaic Virus (WSMV) designated S1RN. An additional infectious clone with a GUS insert was also created to permit easy observation of virus movement (Gus1RN). A single point mutation made within the HC-Pro region created a mutant of WSMV designated PS81 that was unable to cause a systemic viral infection, although it could i...