نتایج جستجو برای: whole gut homogenate
تعداد نتایج: 333278 فیلتر نتایج به سال:
OBJECTIVE To investigate whether a whole grain diet alters the gut microbiome and insulin sensitivity, as well as biomarkers of metabolic health and gut functionality. DESIGN 60 Danish adults at risk of developing metabolic syndrome were included in a randomised cross-over trial with two 8-week dietary intervention periods comprising whole grain diet and refined grain diet, separated by a was...
A soluble phospholipase C (PLC) from boar sperm generates InsP(3) and hence causes Ca(2+) release when added to sea urchin egg homogenate. This PLC activity is associated with the ability of sperm extracts to cause Ca(2+) oscillations in mammalian eggs following fractionation. A sperm PLC may, therefore, be responsible for causing the observed Ca(2+) oscillations at fertilization. In the presen...
We describe a method for estimating the total activity of 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) reductase (EC 1.1.1.34) in the small intestine of rats. An homogenate of the whole small intestine is prepared rapidly and assayed directly to maximize the yield of enzyme and to minimize opportunity for uncontrolled change in activity. Fresh homogenate inhibits the expression of reductase in hepa...
We examined whether changes in the gut microbiota induced by clinically relevant interventions would impact the bioavailability of dietary amino acids in neonates. We tested the hypothesis that modulation of the gut microbiota in neonatal pigs receiving no treatment (control), intravenously administered antibiotics, or probiotics affects whole body nitrogen and amino acid turnover. We quantifie...
In several recent publications (14) it has been reported that dietary aminopterin produces a marked decrease in rat liver choline oxidase activity as measured by the whole homogenate technique. A simple folic acid deficiency, however, has been shown to produce only slight changes in the activity of this enzyme (5). It was probable that a simple folic acid deficiency did not deplete the tissues ...
There has been a dramatic increase in obesity, which presents a risk of many chronic health problems. Recent studies have shown that obese individuals harbor an abnormal gut microbiota (dysbiosis), which has sparked interest in the gut microbiota as a target for weight management. Dietary fiber and whole grains that can be metabolized by gut microbiota have been shown to promote the growth of b...
A tube-feeding model for administering microplastic (MP, Ø = 30 ?m) spheres to fish larvae was employed quantify the uptake of hydrophobic organic contaminants (HOCs) into larval body through a single administration MP. Polychlorinated biphenyl-153 (PCB-153) used as representative HOC that can be sorbed MP in sea. Atlantic herring (Clupea harengus) (34–51 days post-hatching) were selected anima...
OBJECTIVE Pneumonia accounts for more deaths than any other infectious disease worldwide. The intestinal microbiota supports local mucosal immunity and is increasingly recognised as an important modulator of the systemic immune system. The precise role of the gut microbiota in bacterial pneumonia, however, is unknown. Here, we investigate the function of the gut microbiota in the host defence a...
Quantitative real-time PCR was employed to measure the Solenopsis invicta virus 1 (SINV-1) load in tissues, individuals, and among colonies of the red imported fire ant, S. invicta Buren. Among tissues examined from SINV-1-infected adults and larvae, the alimentary canal (specifically the midgut) consistently had the highest number of SINV-1 genome copies (91.1 and 99.9%, respectively). Negativ...
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