Eukaryotic protein synthesis initiation factor (eIF)-4C was purified from wheat germ and the molecular weight was calculated to be approximately 19,000 by SDS-polyacrylamide gel electrophoresis. A similar molecular weight was determined by gel filtration chromatography indicating that wheat germ eIF-4C is functional as a single polypeptide chain. An efficient in vitro translation system depende...

The lectin binding characteristics of goldfish retinal explants were examined by fluorescence microscopy. Neurites grown out from cultured retinal explants were found to bind concanavalin A, wheat germ agglutinin and ricin (agglutinin). The effects of tissue fixation on lectin binding to retinal explant neurites suggest that glycolipids may constitute the predominant ricin binding sites. A redu...

Previous studies showed that murine polymorphonuclear leukocytes (PMNs) lyze tumour cells in the presence of wheat germ agglutinin or actinomycin D. This paper reports studies on whether a soluble factor participates in PMN-mediated cytolysis dependent on lectin or a chemotherapeutic drug. Tumour lysis was observed with supernatants from PMN cocultured with wheat germ agglutinin or actinomycin ...

Nuclei isolated from rat liver, when incubated with methyl-labeled S-adenosylmethionine, incorporated label into 2'-O-methyladenosine, 2'-O-methylguanosine, 2'-O-methyluridine, and 2'-O-methylcytidine of endogenous nuclear RNA. Addition of ribosomal RNA from wheat germ to the reaction markedly stimulated 2'-O-methylation of total RNA. The relative incorporation of label into the four different ...

With the use of the cytochemical stain for horseradish peroxidase of Graham and Karnovsky (1966. J. Histochem. Cytochem.14:291), conjugates of horseradish peroxidase with ricin, wheat germ agglutinin, and phytohemagglutinin were employed for the morphologic demonstration of d-galactose (ricin), N-acetylglucosamine (wheat germ), and N-acetylgalactosamine (phytohemagglutinin) containing moieties ...

the use of proteins and peptides as human therapeutics has been increased in recent years. lipase is a relatively homogeneous proteinaceous enzyme indicated in maldigestion. the purpose of the present study was to evaluate the effect of humidity along with compactional pressure on the enzymatic activity of wheat germ lipase. samples of lipase powder were kept at different relative humidity (rh)...

1. Poly(A)-containing RNA was isolated from the nuclei of mammary gland, liver and brain of lactating guinea pigs. 2. Total nuclear poly(A)-containing RNA from mammary gland inhibited mRNA-directed protein synthesis by a wheat-germ cell-free system. It also inhibited the endogenous activity of the wheat-germ and other cell-free systems. It did not inhibit a wheat-germ cell-free system directed ...

Most of the wheat germ in cereal grains is removed during the milling process. Various physiological effects have been reported for bioactive substances in wheat germ such as phenolic acids and flavonoids. In this study, the anti-oxidant and anti-adipogenic effects of ethanol extracts from wheat germ (WGE) and wheat germ fermented with Aspergillus oryzae (F-WGE) were investigated in HepG2 and 3...

1. Groups of adult colostimized chickens were given diets with and without dietary fibre in the form of bagasse or wheat straw. The fibrous materials were analysed for their contents of cellulose, hemicellulose and lignin. The digestible energies (DE) and metabolizable energies (ME) of these diets were measured by a balance method. 2. Groups of germ-free and conventional chicks aged 4 weeks wer...