Rapid and economical screening of plant pathogens is a high-priority need in the seed industry. Crop quality control and disease surveillance demand early and accurate detection in addition to robustness, scalability, and cost efficiency typically required for selective breeding and certification programs. Compared to conventional bench-top detection techniques routinely employed, a microfluidi...

In August 1946, marked viruslike symptoms were observed on watermelon {Citrullus vulgaris Schrad.) and muskmelon (Cucumis melo L.) plants in a field at Merrimac, Wis. Inoculations from infected plants to tobacco (Nicotiana tabacum L.), cucumber (Cucumis sativus i.)> ö.nd watermelon demonstrated that the disease was of virus origin. Isolates from watermelon and muskmelon were identical, and one ...

Tomato mottle mosaic virus was recently reported from the United States following its original description from Mexico as a novel Tobamovirus species. We present the first complete genome sequence of a tomato mottle mosaic virus isolate from the United States.

background: potyviruses are accounted for 40% of viral diseases of various crops including vegetables and legumes. potyviruses can be transmitted through plant sap, seeds and many aphid species. due to ease of spread of these viruses detection of such viruses are crucial as to the control of the incited diseases. objectives: this study compared the efficiencies of two couples of primers in thei...

Pathogens, including tospoviruses, are known to manipulate the behaviour of vectors after virus acquisition by plants enhance transmission. Furthermore, as recently proven in maize chlorotic mottle pathosystem, vector's choice for virus-infected can change a preference noninfected uptake vector. A similar trend was observed cowpea - Watermelon Bud Necrosis Virus (WBNV) Thrips palmi (Karny) path...

The initiation site for reconstitution on genome RNA was determined by electron microscopic serology for a watermelon strain of cucumber green mottle mosaic virus (CGMMV-W), which is chemically and serologically related to tobacco mosaic virus (TMV). The initiation site was located at the same position as that of the cowpea strain, a virus that produces short rods of encapsidated subgenomic mes...