The water-soluble crude polysaccharide (DDP) obtained from the aqueous extracts of the stem of Dendrobium denneanum through hot water extraction followed by ethanol precipitation, was found to have an average molecular weight (Mw) of about 484.7 kDa. Monosaccharide analysis revealed that DDP was composed of arabinose, xylose, mannose, glucose and galactose in a molar ratio of 1.00:2.66:8.92:34....

A water-soluble crude polysaccharide (DFHP) obtained from the aqueous extracts of the stem of Dendrobium fimhriatum Hook.var.oculatum Hook through hot water extraction followed by ethanol precipitation, was found to have an average molecular weight (Mw) of about 209.3 kDa. Monosaccharide analysis revealed that DFHP was composed of mannose, glucose and galactose in a content ratio of 37.52%; 43....

Sequential treatments of dewaxed Calamagrostis angustifolia Kom with water (60 oC and 90 oC), 70% ethanol, and 70% ethanol containing 0.2%, 1.0%, 2.0%, 4.0%, and 8.0% NaOH at a solid to liquid ratio of 1:25 (g/mL) at 80 oC for 3 h yielded 36.2% soluble polysaccharides of the dry dewaxed material. The eight polysaccharide fractions obtained were comparatively studied by sugar analysis, GPC, FT-I...

The disc gel electrophoretic patterns obtained with skin test-active (mycelial and yeast) antigens of Blastomyces dermatitidis were compared. As would be expected, the blastomycins (mycelial-phase) and the cytoplasmic ultrafiltrates (yeast-phase) were heterogeneous mixtures containing proteins, glycoproteins, lipoproteins, and carbohydrates. The skin test-active cytoplasmic ultrafiltrates and t...

Juvenile American white shrimp (Litopenaeus vannamei) were immersed in aerated beta-glucan and sulphated polysaccharide solutions for 1, 3 and 6 h. Superoxide anion and SOD activity in haemocytes and muscle were investigated to evaluate whether beta-glucan and sulphated polysaccharide induce any immunostimulatory activity. Haemocytes and muscle showed different levels of superoxide anion genera...

The water-soluble polysaccharide fraction from thaeilaria verrucesa (GWS) has been reported to increase the phagocytic actiyity of mice [Yoshizawa et alL, ?Vimpon Shokuhin Kbgye Gakkut'sili, 41 557-560 (GWS-E) was (1994)]. In this study, the macrophage-stimulation activity of enzyrnediegraded GWS inyestigatedbyintraperitoneal]yandorallyadministeringGVVS-Etomice.Theintraperitonealadministration ...

a water soluble polysaccharide, hb-1, with a molecular weight of 23,930, was isolated from radix ranunculi ternati. by hot water extraction, ethanol precipitation, deproteination，ultrafiltration and gel-filtration column chromatography. its sugar composition was determined by glc as glc, ara, and gal in a molar ration of 16.071: 2.722: 1. and the absolute configuration of glc was identified as ...

A water-soluble polysaccharide from Sarcodia ceylonensis was obtained by using the method of water-extraction and ethanol-precipitation. The polysaccharide was further purified by chromatography on AB-8 and ADS-7 columns, yielding a pure polysaccharide termed SCP-60. The molecular weight (Mw) of SCP-60 was calculated to be 50.0 kDa, based on the calibration curve obtained with a series of Dextr...

A water-soluble complex containing ergosterol together with a component of yeast has been isolated. The complex can be isolated from commercial yeast extract to which ergosterol has been added or directly from whole yeast cells. The complexing component has the properties of a large polysaccharide and the binding between the sterol and the polysaccharide appears to be noncovalent. The complex i...