The mechanism of morphological phase transitions was studied for rod-shaped supramolecular assemblies comprised of a poly(acrylic acid)-block-poly(methyl acrylate)-block-polystyrene (PAA(90)-b-PMA(80)-b-PS(100)) triblock copolymer in 33% tetrahydrofuran/water after perturbation by reaction with a positively charged water-soluble carbodiimide. Tetrahydrofuran solvation of the hydrophobic core do...

The results of a theoretical study of the one-, two- and three-water hydrolyses of carbodiimide and the one- and two-water hydrolyses of methyleneimine are presented. All structures were optimized and characterized at the MP2(full)/6-31G* level of theory. Energies for the one-water hydrolysis of carbodiimide were determined at numerous higher levels of theory, up to the QCISD(T)(fc)/6-311+G(3df...

In our previous work, the effect of use of a water soluble CDI/NHS system as nontoxic cross-linking agent on fabrication of gelatin nanoparticles was investigated. In this research, the effect of variation in some synthetic parameters of gelatin nanoparticles cross-linked by CDI/NHS system such as type of gelatin and formulation of cross- linking agent on their size and distribution was examine...

The reaction of the water-soluble carbodiimide, lethyl-3(3-dimethylaminopropyl)carbodiimide, @DC) with bovine lutropin (bLH) has been studied. Increasing the concentration of EDC from 0.01 M to 0.1 M increased the extent of covalent cross-linking between the 1y and p subunits of bLH with a concomitant decrease in receptor-binding activity of the nondissociable product from 40% (0.01 M EDC) to 5...

A hypothetical model of the cytochrome c peroxidase.cytochrome c complex (Poulos, T. L., and Kraut, J. (1980) J. Biol. Chem. 255, 10322-10330) predicts charge interactions between aspartic acid residues of the peroxidase having a spatial distribution that is complementary to the distribution of essential and highly conserved residues of cytochrome c. In a first attempt to test this model, carbo...

A novel crosslinking method was adopted to modify the porous collagen scaffolds by using a water-soluble carbodiimide, 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDAC) and N-hydroxysuccinimide (NHS) in the presence of lysine, which functions as a crosslinking bridge. In vitro biodegradation tests proved that in the presence of lysine the biostability of the EDAC crosslinked scaffolds was g...

Uniform and crystalline nanofibers of perylene-3,4,9,10-tetracarboxylic dianhydride (PTCDA), an insoluble organic semiconducting molecule, have been achieved by self-assembling the molecules using chemical reaction mediated conversion of an appropriately designed soluble precursor, perylene tetracarboxylic acid (PTCA) using carbodiimide chemistry.