this study focused on hepatosomatic and gonadosomatic index and levels of plasma 17β-estradiol (e2), vitellogenin (vtg) and total protein and determined the localization of vtg in the liver during vitellogenesis in female chalcalburnus tarichi, an endemic cyprinid species living in the lake van, turkey. the levels of plasma e2, vtg and total protein in female fish increased during vitellogenesi...

We characterized dynamics of the plasma yolk precursors vitellogenin (VTG), very-low-density lipoprotein (total VLDL-TG), and VLDL particle size distribution during egg production by female greater scaup (order: Anseriformes, Aythya marila). We also evaluated VTG and total VLDL-TG as physiological indices of reproductive state. Mean (+/-1 SE) plasma concentrations of VTG and total VLDL-TG for f...

The gene, vitellogenin (vtg) was cloned and characterized in the dojo loach (Misgurnus anguillicaudatus), an indigenous freshwater species in East Asia, in order to develop tools for detecting the effects of estrogenic endocrine-disrupting chemicals (EEDCs). Full-length cDNAs encoding seven distinct vtg transcripts (vtg1-7) were obtained. The corresponding deduced amino acid sequences (Vtg1-7) ...

To examine the behavior of the estrogenic biomarker vitellogenin (VTG) under the combined impact of estrogens and pathogens, parasite-infected or noninfected rainbow trout were exposed to two doses of 17β-estradiol (E2). Infected and E2-exposed fish showed significantly lower hepatic VTG mRNA levels than healthy fish. Transcriptome data suggest that this was due to energetic constraints. Reduce...

three complete cdnas encoding different forms of vitellogenin (vtg) were cloned from a white perch (Morone americana) liver cdna library. the two longest cdnas encoded Vtgs with a complete suite of yolk protein domains that, based on comparisons with vtg sequences from other species, were categorized as Vtgaa and Vtgab. the shorter cdna encoded a Vtg that lacked a phosvitin domain, had a shorte...

There are increasing evidences that show many xenobiotic chemicals (called as endocrine disruptorchemicals EDCs) through interfering with endocrine system, have the capability to induce developmentaland reproductive abnormalities in humans and animals. The yolk protein precursor vitellogenin (VTG) hasproved to be a simple and sensitive biomarker for assessing exposure of fish to EDCs especially...

Vitellogenin (VTG) is a precursor of egg-yolk protein and is therefore present at high concentrations in the plasma of female fish. In male fish, VTG concentrations are usually undetectable or low, but can be induced upon exposure to oestrogenic substances either via the water or the diet. This work was carried out to determine the reason for apparently elevated VTG concentrations in unexposed ...

Vitellogenin (VTG) is known to be a female-specific protein and a precursor form of egg yolk. Threevitellogenin,Vtg I,Vtg II and Vtg III have been identified in chicken.Vitellinogen precursors supply the major egg yolk proteins which are a source of nutrients during early development of egg-laying (oviparous) vertebrates and invertebrates. A complete structural analysis and 3-D modelling of Vit...

The effects of oestrogenic exposure upon exposure indicators and reproductive success in a marine fish were investigated using a series of short and long-term exposures of the sand goby (Pomatoschistus minutus) to oestrogenic materials. A 140 bp cDNA probe for sand goby vitellogenin (VTG) was used to measure hepatic VTG mRNA expression. Effects on other novel and “classical” biomarkers of oestr...

We monitored the contamination by environmental estrogens (EEs) of coastal areas in Korea and Japan using the wild grey mullet. The grey mullet were collected from Ansan, Jeju, Yeosu, Tongyeong, and Busan in Korea and Nagasaki, Omuta, and Fukuoka in Japan. Contamination by EEs was determined by measuring vitellogenin (VTG) levels in serum and identifying gonadal abnormalities histologically (i....