this study was carried out in order to determine the effects of boron and cold stress on pollen germination of almond hybrids in vitro culture, two different media including the absence and presence of boron (0 mg/l, and 100 mg/l) in 10% sucrose and 1% agar medium at 24°c in dark conditions. the results showed that different treatments had a significant effect on the germination percentage. the...

This study was carried out in order to determine the effects of boron and cold stress on pollen germination of almond hybrids in vitro culture, two different media including the absence and presence of boron (0 mg/l, and 100 mg/l) in 10% sucrose and 1% agar medium at 24°C in dark conditions. The results showed that different treatments had a significant effect on the germination percentage. The...

Neospora caninum is a coccidian protozoan parasite which is a major cause of bovine abortions and neonatal mortality in cattle, sheep, goat and horse. Occasionally, cultured cells are used for isolation and multiplication of the agent in vitro with several purposes. In this study the tachyzoite yields of N. caninum were compared in various cell cultures as the host cell lines. Among the cell cu...

neospora caninum is a coccidian protozoan parasite which is a major cause of bovine abortions and neonatal mortality in cattle, sheep, goat and horse. occasionally, cultured cells are used for isolation and multiplication of the agent in vitro with several purposes. in this study the tachyzoite yields of n. caninum were compared in various cell cultures as the host cell lines. among the cell cu...

Background Endometriosis is the presence of endometrial tissue outside of the uterine cavity and is the most common gynecologic disorder in women of reproductive age. We have preliminary evidence that in the presence of a 3-dimensional (3-D) fibrin matrix, human endometrial glands, stroma, and neovascularization can develop in vitro, mimicking the earliest stages of endometriosis. The aim of th...

background: endometriosis is the presence of endometrial tissue outside of the uterine cavity and is the most common gynecologic disorder in women of reproductive age. we have preliminary evidence that in the presence of a 3-dimensional (3-d) fibrin matrix, human endometrial glands, stroma, and neovascularization can develop in vitro, mimicking the earliest stages of endometriosis. the aim of t...

To develop an alternative method for production of sarsasapogenin by plant tissue culture technique and its quantification in Asparagus racemosus and its in vitro cultures using HPTLC. Murashige and Skoog’s (MS) basal medium supplemented with various growth regulators was used for development of shoot and root calli while, hormone free MS medium was used for development of Agrobacterium tumefac...

introduction: the spermatogonial stem cells (sscs) form the basis of spermatogenesis. in vitro culture of germ cells is useful for the purification and increasing of sscs for transplantation. in the present study, we examined: in vitro the effects of co-culture with sertoli cells, gdnf, scf and gm-csf on efficiency of spermatogonial cells colony formation and spermatogenesis induction in the re...