A new method for determining the vitrification rate of pottery depending on the firing temperature was devised using secondary electron images (SEI) of scanning electron microscope (SEM). Several tests were performed to establish the appropriate operating conditions of SEM and reproducibility as well as to examine the applicability of the method. The grayscale values converted from each pixel o...

In the last decades significant advances have been made in successful cryopreservation of mammalian oocytes. Human oocyte cryopreservation has practical application in preserving fertility for individuals at risk of compromised egg quality due to cancer treatments or advanced maternal age. While oocyte cryopreservation success has increased over time, there is still room for improvement. Oocyte...

OBJECTIVE This study was designed to investigate the survival rate of vitrified mouse blastocysts depending on the presence or absence of sucrose in vitrification solution. METHODS Mouse two-cell embryos were collected and cultured to blastocysts. Two vitrification solutions were prepared. The control solution was composed of 25% glycerol, 25% ethylene glycol, and 0.5 M sucrose (G25E250.5S) c...

Our aim was to optimize the cryoprotectant treatment for the preservation of immature porcine cumulus-oocyte complexes (COCs) by solid surface vitrification. In each experiment, the vitrification solution consisted of 50 mg/ml polyvinyl pyrrolidone, 0.3 M of the actual sugar and in total 35% (v/v) of the actual permeating cryoprotectant (pCPA) combination. After warming, the COCs were subjected...

T he emerging field of tissue engineering has identified product storage as a significant obstacle for commercialization of products containing living cells. Traditionally employed methods of tissue banking utilize cryopreservation by freezing. Unfortunately, cryopreservation by freezing may result in loss of tissue function and viability by several recognized mechanisms of which ice formation ...

background: routine oocytes cryopreservation remained an elusive technique in the wide ranges of available assisted reproductive technologies. the microtubules of oocytes are vulnerable to cryoprotectants and thermal change during cryopreservation. objective: the effects of a vitrification protocol were investigated on the spindle and chromosome configurations of mice oocytes cryopreserved at t...

OBJECTIVE To evaluate and compare the laboratory and clinical outcomes of vitrification with slow-freezing method for cryopreservation of embryos and blastocysts in an in-vitro fertilisation programme. DESIGN Retrospective analysis of all the 104 cycles of frozen embryo and blastocyst replacements from 2003 to 2008 and all the 149 cycles with embryos or blastocysts for vitrification from 2006...

BACKGROUND The traditional vitrification method cannot keep up with the increased culture and propagation efficiency required to cryopreserve large quantities of vigorously proliferating human embryonic stem (HES) cells. In this study, we describe a newly invented vitrification carrier for cryopreserving large amount of HES cells and evaluate whether this bulk vitrification (BV) method is as ef...

Cryopreservation is the most promising way for long-term storage of biological samples e.g., single cells and cellular structures. Among various cryopreservation methods, vitrification is advantageous by employing high cooling rate to avoid the formation of harmful ice crystals in cells. Most existing vitrification methods adopt direct contact of cells with liquid nitrogen to obtain high coolin...

Ice-free cryopreservation, referred to as vitrification, is receiving increased attention in the human and animal assisted reproduction. However, it introduces the detrimental osmotic stress by adding and removing high contents of cryoprotectants. In this study, we evaluated the effects of normalizing cell volume regulation by adding glycine, an organic osmolyte, during vitrification of mouse g...