Herein, we presented the first report on bluetongue (BT) disease in 10 pregnant camels in a herd fromKerman province, Iran. All sera samples were tested serologically (AGID, C-ELISA). We also used the Razi-BK cell line, performed primary culture of ovine kidney and inoculated intravenously the embryonatedchicken eggs (ECE) to culture and isolate the BT virus. Efforts to culture and isolation of...

PROPAGATION OF LOW PATHOGENICITY AVIAN INFLUENZA VIRUSES by KIRA ANN MORESCO (Under the Direction of David E. Swayne) ABSTRACT Growth parameters of low pathogenicity avian influenza viruses were examined in eight cell cultures and in three types of avian embryos. Virus detection was compared for samples collected from experimentally infected birds, using real-time reverse transcriptase polymera...

Detection of bovine viral diarrhea virus in 143 blood samples by virus isolation in cell culture and flow cytometry was performed. The material included 37 samples later shown to originate from persistently infected cattle. Thirty-three samples were positive by virus isolation, and all 37 samples were positive by the flow cytometric assay.

The 2006 mumps resurgence in the United States raised questions about the appropriate isolation period for people with mumps. To determine the scientific basis for isolation recommendations, we conducted a literature review and considered isolation of virus and virus load in saliva and respiratory secretions as factors that were related to mumps transmission risk. Although mumps virus has been ...

Experimental infection of Muscovy ducks with V4 strain of Newcastle disease virus was undertaken to determine the response of the ducks to the virus and the possibility of virus transmission to ducks and chickens in village like conditions. Twelve ducks were randomly and equally divided into three groups of control, inoculated and in-contact. Additionally, the chickens were placed into two grou...

Infectious laryngotracheitis (ILT) disease is an acute highly contagious viral disease leading to massive economic losses to the national poultry industry. This study aimed to identify the most accurate and rapid diagnostic methods to rescue layer poultry farms from intense outbreaks in Egypt. Fifty pathological specimens were collected and subjected to virus isolation (VI), histopathology, dir...

Direct immunofluorescence (IF) staining was compared with virus isolation for detection of herpes simplex viruses (HSV) and varicella-zoster virus (VZV) directly in clinical materials. These included 199 vesicular lesion specimens and 280 tissue specimens. Correspondence between IF and isolation results was 88% in testing for HSV in lesion specimens and 98% in testing for HSV in various tissue ...