Because many viruses are readily amenable to molecular, genetic, and biochemical analyses, and because the replication of certain viruses has been the target of antiviral drug development, a wealth of information has accumulated regarding the structure, functions, properties, and regulation of eukaryotic viral DNA polymerases. Viral DNA polymerases have interesting similarities with eukaryotic ...

The Epstein-Barr virus (EBV) lytic program includes lytic viral DNA replication and the production of a viral particle into which the replicated viral DNA is packaged. The terminal repeats (TRs) located at the end of the linear viral DNA have been identified as the packaging signals. A TR-negative (TR(-)) mutant therefore provides an appropriate tool to analyze the relationships between EBV DNA...

The key DNA cutting and joining steps of retroviral DNA integration are carried out by the viral integrase protein. Structures of the individual domains of integrase have been determined, but their organization in the active complex with viral DNA is unknown. We show that HIV-1 integrase forms stable synaptic complexes in which a tetramer of integrase is stably associated with a pair of viral D...

The disease is caused by members of the gammaherpesviruses group. Alcelaphine herpesvirus-1 (AHV-1), carried asymptomatically by the wildebeest (Connochaetes taurinus) in Africa, has been diagnosed in disease outbreaks in zoos in North America. Ovine herpesvirus-2 (OHV-2) is carried asymptomatically by sheep (117). A third, as yet unnamed virus, has been detected in an outbreak of MCF in white-...

In situ hybridizations show that 5 min after parasitization, polydnavirus DNA is in close vicinity of the parasitoid egg, but 5 h later also in the yolk and partially in the host embryo. Fifteen hours after parasitization, the viral DNA is seen all over the host embryo and hardly in the yolk. The tissue distribution of the viral DNA was analysed and quantified by dot blots in the fifth instar p...

Nucleic acid hybridization studies were made between 71S-AMV-RNA and DNA from leukemic myeloblasts and from normal chicken cells. There was homology between the viral RNA and chicken cell DNA and to a greater extent between viral RNA and leukemic cell DNA. Leukemic cell DNA hybridized approximately twice as much viral RNA as did normal chicken DNA. Thermal melting studies showed that the viral ...

F2408 rat cells transformed by polyoma virus contained integrated and nonintegrated viral DNA. The presence of nonintegrated viral DNA is under control of the A early viral function. Polyoma ts-a-transformed rat cells lose the free viral DNA when growth at the nonpermissive temperature (40 degrees C), but they reexpress it 1 to 3 days after they are shifted back to the permissive temperature. I...

conclusions igm alone is not a marker for viral shedding in genital tract. molecular testing in conjunction of igg test should be evaluated as an option to determine hsv status, and applied for research on hsv genital infections records. methods two hundred and eight females were included in the study; igm antibodies against hsv1/2 were detected in serum samples; the real-time polymerase chain ...