European Bioanalysis Forum Focus Workshop, Lisbon, Portugal, 9-10 June 2016 At the recent European Bioanalysis Forum's Focus Workshop 'Bringing Assay Validation and Analysis of Biomarkers into Practice', the discussion on best practice for biomarker assay validation continued. Both the presentations and the adjacent panel discussions yielded valuable food for thought for the broader bioanalytic...

Because of the importance of biomarkers within medicine as a whole and the increasing realisation that imaging can safely provide biomarkers, the ESR executive commissioned this report by experts in the field. It is hoped that further awareness amongst our community will contibute to further exploitation of the enormous wealth of biomaker information available in our day to day imaging. The all...

We present results from a genomewide association study (GWAS) and a single-marker association study. The GWAS was performed with the Illumina PorcineSNP60 BeadChip from which 5 markers were selected for a validation analysis. Genetic effects were estimated for feed intake, weight gain, and traits of fat and muscle tissue in German Landrace boars kept on performance test stations. The GWAS was p...

Problem statement: The presence of metastasis in the regional lymph nodes is the most important factor in predicting prognosis in breast cancer. Many biomarkers have been identified that appear to relate to the aggressive behaviour of cancer. However, the nonlinear relation of these markers to nodal status and also the existence of complex interaction between markers have prohibited an accurate...

Proper analysis and validation of gene expression data is not a trivial task. Most of the existing approaches identify individual or group of markers/signatures where the validation only goes as far as literature and biological experimental validation. In this paper we discuss a framework that could be considered to develop and validate patterns from gene expression data and work towards future...

PURPOSE Discriminant markers for pancreatic cancer detection are needed. We sought to identify and validate methylated DNA markers for pancreatic cancer using next-generation sequencing unbiased by known targets. EXPERIMENTAL DESIGN At a referral center, we conducted four sequential case-control studies: discovery, technical validation, biologic validation, and clinical piloting. Candidate ma...

Interest in genetic improvement of carcass and tenderness traits of beef cattle using genome-based selection (GS) and marker-assisted management programs is increasing. The success of such a program depends on the presence of linkage disequilibrium between the observed markers and the underlying QTL as well as on the relationship between the discovery, validation, and target populations. For mo...

The current generation of biologic markers have three characteristics that differentiate them from previous ones. These include the ability to detect xenobiotics at concentrations at the cellular and molecular level, to detect earlier biologic changes presumptive of disease or disease risk, and to identify a detailed continuum of events between an exposure and resultant disease. If biomarkers a...

Myocardial infarction (MI) is one of the leading causes of mortality and morbidity worldwide. Although several markers are identified associated with MI, early diagnostic or predictive markers are yet to be identified. Using an integrative bioinformatics approach, here we have reported 18 key genes and mong which seven could be associated with early MI pathogenesis. Expression signature of thes...