نتایج جستجو برای: using rep

تعداد نتایج: 3393050  

پایان نامه :وزارت علوم، تحقیقات و فناوری - دانشگاه صنعتی اصفهان - دانشکده ریاضی 1390

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Journal: :Journal of molecular biology 2001
W Cheng J Hsieh K M Brendza T M Lohman

E. coli Rep protein is a 3' to 5' SF1 superfamily DNA helicase which is monomeric in the absence of DNA, but can dimerize upon binding either single-stranded or duplex DNA. A variety of biochemical studies have led to proposals that Rep dimerization is important for its helicase activity; however, recent structural studies of Bacillus stearothermophilus PcrA have led to suggestions that SF1 hel...

Journal: :Journal of virology 2003
Yiguo Hong John Stanley Rene van Wezel

The origin of replication of African cassava mosaic virus (ACMV) and a gene expression vector based on Potato virus X were exploited to devise an in planta system for functional analysis of the geminivirus replication-associated protein (Rep) in transgenic Nicotiana benthamiana line pOri-2. This line contains an integrated copy of a tandem repeat of the ACMV origin of replication flanking nonvi...

2015
Zainul A. Khan Malik Z. Abdin Jawaid A. Khan

Cotton leaf curl Burewala virus (CLCuBuV), belonging to the genus Begomovirus, possesses single-stranded monopartite DNA genome. The bidirectional promoters representing Rep and coat protein (CP) genes of CLCuBuV were characterized and their efficacy was assayed. Rep and CP promoters of CLCuBuV and 35S promoter of Cauliflower mosaic virus (CaMV) were fused with β-glucuronidase (GUS) and green f...

Journal: :Nucleic acids research 1998
S A Akbar Behjatnia I B Dry M Ali Rezaian

The geminiviral replication-associated protein (Rep) is the only viral protein required for viral DNA replication. Tomato leaf curl virus (TLCV) Rep was expressed in Escherichia coli as a histidine-tagged fusion protein and purified to homogeneity in non-denaturing form. The fusion protein was used in in vitro binding experiments to identify the Rep-binding elements within the origin of replica...

Journal: :MONET 2010
Albert Wasef Xuemin Shen

It is well recognized that security is vital for reliable operation of vehicular ad-hoc networks (VANETs). Location privacy is one of the main security challenges in VANETs, which is concerned with preventing an attacker from tracking a specific vehicle. In this paper, we propose a novel location privacy preservation scheme for VANETs using random encryption periods (REP). REP is based on a pri...

Journal: :American journal of physiology. Heart and circulatory physiology 2004
Lynne L Johnson Lorraine Schofield Mark Bouchard Leonard Chaves Athena Poppas Steven Reinert Paul Zalesky Jeffrey Creech David O Williams

This study was designed to test the hypothesis that raising myocardial O2 via diffusion of a hyperbaric oxygen solution (AO) administered through the anterior interventricular vein (AIV) will reduce infarct size by reducing reperfusion injury associated with reduced neutrophil activation. In three pilot open-chest swine experiments, myocardial tissue Po2 was monitored using an oxygen probe duri...

Journal: :Journal of immunology 2010
Yufeng Li Shujuan Liu Jessica Hernandez Luis Vence Patrick Hwu Laszlo Radvanyi

We determined how CD8(+) melanoma tumor-infiltrating lymphocytes (TILs) isolated from two distinct phases of expansion in preparation for adoptive T cell therapy respond to melanoma Ag restimulation. We found that TILs isolated after the rapid expansion protocol (REP) phase, used to generate the final patient TIL infusion product, were hyporesponsive to restimulation with MART-1 peptide-pulsed ...

2014
Jin-Sub Kim Sungae Cho Kyoung-Hwan Joo Joon-Sang Lee Daniel H. Conrad Sung-Weon Cho

Background: IgE is closely related to the development of allergies. However, the poor relationship between the specific IgE level and the severity of allergic diseases suggests the possibility of functionally different IgE isoforms. With this in mind, rat basophilic leukemia (RBL)-2H3 activation was analyzed with each type of rat IgE for two parameters, exocytosis and IL-4 mRNA production. RBL-...

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