نتایج جستجو برای: using qrt
تعداد نتایج: 3392536 فیلتر نتایج به سال:
Lentinula edodes (shiitake mushroom) is a common edible mushroom with a number of potential therapeutic and nutritional applications. It contains various medically important molecules, such as polysaccharides, terpenoids, sterols, and lipids, were contained in this mushroom. Quantitative real-time polymerase chain reaction (qRT-PCR) is a powerful tool to analyze the mechanisms underlying the bi...
PURPOSE Sentinel node biopsy (SNB) has been shown to accurately stage the regional lymphatics in oral carcinoma. However, intraoperative pathology is only moderately sensitive and final pathology takes several days to complete. The purpose of this study was to develop a rapid, automated, and quantitative real-time PCR (qRT-PCR) assay that can match final pathology in an intraoperative time fram...
تغذیه گیاهخواران موجب فعال شدن سیستم دفاع مستقیم و غیرمستقیم در گیاه میشود. بهمنظور بررسی نقش تعدادی از ژنهای مؤثر در دفاع مستقیم و یا غیرمستقیم لوبیا در برابر کنه تارتن دولکهای، میزان بیان این ژنها در تعدادی از ژنوتیپهای حساس (ژنوتیپ اختر) و مقاوم (ناز و ks41128) لوبیا به کنه دولکهای، قبل و بعد از آلودگی به کنه دولکهای بوسیله qrt-pcrمقایسه شد. سپس بهمنظور تأیید نتایج آزمایش qrt-pcr، ...
in this study, several designed creb1-specific shrnas cloned into lentiviral transfer vectors were screened in293t by transient transfection assays. relative creb1 expression was determined by relative qrt-pcr. all of the designed shrnas gave some level of knockdown. one shrna was more effective for knocking down the specific gene among others. the predicted sirnas activities as determined by r...
vaccines is considered as a critical point and in-process of quality control (ipqc) test of vaccine production. rapid tests, like real time pcr, are more appropriated when the production occurs at industrial scale because of the amounts of starting materials and the excess of consumed time required. in the current study, a real-time quantitative reverse transcription-polymerase chain reaction (...
Background: Acinetobacter baumannii is an important pathogen in health care and is responsible for severe nosocomial and community-acquired pneumonia. To design novel therapeutic agents, a mouse model for A. baumannii pneumonia is essential. Methods: We described a mouse model of A. baumannii using clinical and 19606R standard strains for developing a quantitative real-time PCR (qRT-PCR) for ra...
Results Both WT1 antigens induced significant quantities of IFN-g mRNA production after 3 h in the qRT-PCR protocol. Among 18 samples of HLA-A2+, specific responses were detected in 27.8% (5/18) when Db126 peptide, compared to 16.7% (3/18) in the case of WH187 peptide. On the other hand, no detection (0/18) was obtained with ELISPOT, when both WT1 peptides were tested. In four HLA-A2samples nei...
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