Circadian variations of alanine aminopeptidase (EC 3.4.11.2), gamma-glutamyltransferase (EC 2.3.2.2), and N-acetyl-beta-glucosaminidase (EC 3.2.1.30) in urine were studied in 10 healthy children, ages six to 15 years. Urine specimens were collected during 24 h, grouped into four time intervals. Enzymes were measured spectrophotometrically, with automation. These enzymes all showed diurnal varia...

BACKGROUND Several studies suggested that women may be more susceptible to oxidative damage induced by cigarette smoking, but the role of smoking status and antioxidant capacity in gender difference in susceptibility to oxidative damage has not been well studied. METHODS We conducted a cross-sectional analysis of the baseline data from 146 current and former heavy smokers enrolled in a chemop...

A Gel exclusion chromatographic method was used to determine the molecular weight distribution, and therefore, the origin, of non-dialysable urinary glucoconjugates in normal men. The results showed a mixture of glucoconjugates with molecular weight ranges of 1605 to 141,000. It is suggested that the high molecular weight forms originally constitute the glucoconjugates, and that they are probab...

This work describes a novel method of urinary oxalate determination based on a catalase model compound MnL(H2O)2(ClO4)2 (L 1⁄4 bis(2-pyridylmethyl)amino)propionic acid). Urinary oxalate is first decomposed by the oxalate oxidase into carbon dioxide and hydrogen peroxide. The later is then disproportionated into water and oxygen by the catalase model compound forming a color compound, which can ...

l-fucose (6.deoxy. l.galactose) is the only monosacharide which is placed in non reducting position of glycoproteins structure in l-deoxy form. l-fucosidase and fucosy 1 transferase directly take part in the elevation or decrease of sorum l-fucose levels. tumors may directly induce enzymes that release l-fucose from glycoproteins formed in the neoplastic tissue, or the glycoproteins may be rele...

OBJECTIVES In recent years, there has been an increasing amount of study on early diagnosis of kidney injury through sensitive and specific biomarkers. We examined the practical applicability of the urinary levels of NAG (N-acetyl-β-D-glucosaminidase), AP (alkaline phosphatase), and LDH (lactate dehydrogenase) as renal dysfunction screening biomarkers in full and pre-term newborns treated with ...

Total protein (TP), albumin (Alb), transferrin (TRF), retinol-binding protein (RPB), N-acetyl-beta-glucosaminidase (NAG), alanine aminopeptidase (AAP), gamma-glutamyltransferase (GGT), and creatinine (Cr) were measured in random (untimed) urine samples from 29 nonpregnant women and from pregnant subjects (11 in the first trimester, 34 in the second, and 37 in the third). The excretion of TP, Al...

A partially purified oxalate oxidase from leaves of 10-day-old seedlings of forage sorghum was immobilized covalently onto alkylamine glass beads affixed on inner base of a glass beaker. The enzyme retained 5.17% of its initial activity with a conjugation yield of 182 mg/g support. After immobilization, the enzyme showed an increase in its optimum pH and Km for oxalate but slight decrease in in...

Plasmid transfer via the transformation pathway of Streptococcus pneumoniae was weakly restricted by the DpnI or DpnII restriction endonuclease, either of which gave a reduction only to 0.4, compared with phage infection, which was restricted to 10(-5). The greater sensitivity of plasmid transfer compared with chromosomal transformation, which was not at all restricted, can be attributed to par...

The induction of phase 2 detoxifying enzymes, such as UDP-glucuronosyltransferases (UGTs), in response to an array of naturally occurring and synthetic agents, such as oltipraz (4-methyl-5-[2-pyrazinyl]-1,2-dithiole-3thione), provides an effective means of protection against a variety of carcinogens. Transcription factor Nrf2 is an essential regulator of the inducible expression of detoxifying ...