Normal mouse livers have been perfused in sifu and the release of triglycerides in the perfusate has been measured as an index of very low density lipoprotein secretion. Vincristine or colchicine, drugs known to interfere with the microtubular system, were found to inhibit markedly triglyceride release by perfused livers. When used at appropriate concentrations these drugs did not change glucos...

This study examines the relationship between impaired fatty acid oxidation and the pathogenesis of Reye syndrome. We present a hypothesis proposing that many clinical signs of this childhood disease are caused by accumulation of unusual acyl CoA esters, precursors to deacylated metabolites found in the patients' blood and urine. A new method was developed to measure acyl CoA compounds in small ...

Hyperammonaemia due to a deficient urea cycle (Fig. 1) has been attributed to at least four distinct enzyme defects: (1) hyperanmmonaemia due to a defect of carbamyl-phosphate synthetase L. the liver (Freeman et al., 1964); (2) hyperammonaemia due to a defect of ornithine carbamyl transferase with or without defect of carbamyl phosphate synthetase in the liver (Russell et al., 1962; Hopkins et ...

fact that Munro's experiments were carried out under less satisfactory conditions; he himself states that more than half the tadpoles died during the first week, and that from the original 250 eggs only 10 survived to metamorphosis. Our own animals were well fed on a high-protein diet and had a high survival rate. Munro, on the other hand, does not state that his animals were fed but only that ...

The catabolism of glycine in the isolated perfused rat liver was investigated by measuring the production of 14CO2 from [1-14C]- and [2-14C]glycine. Production of 14CO2 from [1-14C]glycine was maximal as the perfusate glycine concentration approached 10 mM and exhibited a maximal activity of 125 nmol of 14CO2 X g-1 X min-1 and an apparent Km of approximately 2 mM. Production of 14CO2 from [2-14...

I . Metabolic alterations related to protein catabolism were studied in rats in transitional states induced by changing from a low-protein (LP) (so g caseinlkg) diet to a high-protein (HP) (250 g casein/kg) diet. 2. Twenty-four hours after the diet was changed, the rats showed a more rapid increase in live-weight gain than controls that had been fed on the HP diet throughout. On the 5th day aft...

The possible occurrence of a functional ornithine-urea cycle (OUC) and changes of activity of key amino acid metabolism-related enzymes were studied in the amphihaline shad hilsa (Tenualosa ilisha) that were collected from estuarine water of Kakdwip and from freshwater river basin of Bhrahmaputra during the breeding season. Very high concentration of urea was detected in different tissues and p...

1. Inter-organ relationships between glucose, lactate and amino acids were studied by determination of plasma concentrations in different blood vessels of anaesthetized rats fed on either a high-carbohydrate diet [13% (w/w) casein, 79% (w/w) starch] or a high-protein diet [50% (w/w) casein, 42% (w/w) starch]. The period of food intake was limited (09:00-17:00h), and blood was collected 4h after...

The aim of this trial was to evaluate the effect of dietary nucleic acids on ureagenesis and nitrogen balance in gilthead sea bream juveniles. For that purpose, 5 isonitrogenous (7.25% N) diets were formulated based on fish meal as protein source (FM-control diet) and to partially replace the fish meal nitrogen with two levels of nucleic acids (diets RNA1 and RNA2) or brewers yeast (diets BY1 a...

The paper reports the tissue distribution of the enzyme arginase in three different Cichlids: Tilapia zilli, Sarotherodon galilaeus and Oreochromis niloticus, from the Aiba and Osinmo reservoirs, located in the southwestern Nigeria. The tissues of S. galilaeus showed very high activity of arginase as compared with the other two species. The liver of O. niloticus and the gut of T. zillii showed ...