BACKGROUND/AIM Helicobacter pylori is a major health problem. One of the therapeutic approaches is administration of antibody against H. pylori. The methylotrophic Pichia pastoris is a suitable host for expression of recombinant antibody fragments. The aims of this study were the expression and the evaluation of camelid nanobody in the yeast Pichia pastoris. MATERIALS AND METHODS The camelid-...

prior investigators demonstrated helicobacter pylori as a risk factor of liver diseases. in this study association between h. pylori and hepatocellular carcinoma was investigated. patients and methods totally, 59 specimens of liver were collected from two health care centers affiliated to shahid beheshti university of medical sciences, including 22 specimens of hepatocellular carcinoma, 18 spec...

Detection of Helicobacter pylori after triple therapy is usually carried out by either rapid urease test (RUT), urea breath test (UBT), histology, bacterial isolation, and single round PCR or serological tests. In this study, antral biopsy specimens from 25 patients were tested for H. pylori by RUT, culture, histology, and nested PCR in their antral biopsy specimens before and after treatment. ...

Proteus mirabilis, a gram-negative bacterium associated with complicated urinary tract infections, produces a metalloenzyme urease which hydrolyzes urea to ammonia and carbon dioxide. The apourease is comprised of three structural subunits, UreA, UreB, and UreC, assembled as a homotrimer of individual UreABC heterotrimers (UreABC)(3). To become catalytically active, apourease acquires divalent ...

aim: the purpose of this investigation was to determine the oipa and dupa genes of helicobacter pylori isolates from west of iran; chaharmahalo bakhtiyari region and find their relationship with the severity of the gastroduodenal diseases. background: helicobacter pylori is an organism responsible for many gastroduodenal diseases. studies suggest that genetic diversity in h . pylori virulence f...

Introduction Microorganisms regulate soil nitrogen (N) cycling in cropping systems. However, how microbial functional genes involved N respond to mulching practices is not well known. Methods We collected samples from a spring maize field mulched with crop straw (SM) and plastic film (FM) for 10-year no (CK) the Loess Plateau. Microbial were quantified using metagenomic sequencing. Results Comp...