This study aims to characterize the phosphate buffer and urease enzymes through UV-Vis SEM-EDS absorbance spectra using tungsten as an indicator electrode. The method used is a potentiometric biosensor with enzyme immobilization technique for urea analyte. A small detection range of 10-5-10-4M has been studied PVA-enzyme coated electrodes PVC-KTpClPB. On this basis, researchers increased by ana...

Urease was immobilized on platinum electrode both by chemical binding and electropolymerization.The conductometric urea biosensor thus prepared showed a detection limit of 4.9×10-5 M and linear dynamic range from 4.9×10-5 to 5.8×10-3 M for urea concentration when the enzyme is covalently immobilized on Pt electrodes. Conductometric transducers respo...

Urea is one of the final products of protein metabolism. In clinical analysis and dairy industry urea is a very significant parameter as excess in blood serum from its permissible range causes dysfunction of the kidney. Hence, its analysis is significant and is carried out frequently in various laboratories [1, 2]. Urea is an omnipresent compound present in blood and various organic fluids. It ...

Temperature-dependent zeolite synthesis has revealed a unique surface morphology, surface area and pore size which influence the immobilization of urease on gold electrode supports for biosensor fabrication. XRD characterization has identified zeolite X (Na) at all crystallization temperatures tested. However, N₂ adsorption and desorption results showed a pore size and pore volume of zeolite X ...

The presented work focuses on the synthesis of silica-based macroporous aggregates composed silica nanoparticles using a template-free method. To improve mechanical strength prepared fractal-like aggregates, precursor tetraethyl orthosilicate (TEOS) is used during nanoparticle aggregation. obtained results confirm possibility to tailor pore size distribution (PSD) simply by primary particles va...

Well-aligned zinc oxide (ZnO) nanowire arrays were fabricated on gold-coated plastic substrates using a low-temperature aqueous chemical growth (ACG) method. The ZnO nanowire arrays with 50-130 nm diameters and ~1 μm in lengths were used in an enzymebased urea sensor through immobilization of the enzyme urease that was found to be sensitive to urea concentrations from 0.1 mM to 100 mM. Two line...

We assayed the in vivo activity of the ureases of soybean (Glycine max) embryos by genetically eliminating the abundant embryo-specific urease, the ubiquitous urease, or a background urease. Mutant embryos accumulated urea (250-fold over progenitor) only when lacking all three ureases and only when developed on plants lacking the ubiquitous urease. Thus, embryo urea is generated in maternal tis...