The annual subsidy for the Universal PDS in Tamil Nadu was around 5,000crore, and adding that there was a strong administrative monitoring, vigilance and grievance redresses mechanism operating in the state in respect of UPDS. But the quality of rice distributed through the universal pubic distribution system under the free of cost as on 01 June 2011 scheme was of poor quality and unfit for con...

In this study, a novel universal primer-multiplex-PCR (UP-M-PCR) method adding a universal primer (UP) in the multiplex PCR reaction system was described. A universal adapter was designed in the 5'-end of each specific primer pairs which matched with the specific DNA sequences for each template and also used as the universal primer (UP). PCR products were analyzed on sequencing gel electrophore...

The deep sequencing of 16S rRNA genes amplified by universal primers has revolutionized our understanding of microbial communities by allowing the characterization of the diversity of the uncultured majority. However, some universal primers also amplify eukaryotic rRNA genes, leading to a decrease in the efficiency of sequencing of prokaryotic 16S rRNA genes with possible mischaracterization of...

BACKGROUND MicroRNAs (miRNAs) are small, non-coding RNAs capable of postranscriptionally regulating gene expression. Accurate expression profiling is crucial for understanding the biological roles of miRNAs, and exploring them as biomarkers of diseases. METHODOLOGY/PRINCIPAL FINDINGS A novel, highly sensitive, and reliable miRNA quantification approach,termed S-Poly(T) miRNA assay, is designe...

Assessment of genetic diversity and individual relationships in rice (Oryza sativa) germplasm collections seems to be necessary for future rice breeding program. In order to understand genetic relationships of 30 rice genotypes, nine morphological traits, seven physicochemical properties and twelve RAPD primers were used for study of 30 rice genotypes. Among morphological traits, numbe...

objectives: this study aimed to compare the shear bond strength (sbs) of composite to stainless steel crowns (ssc) using two mechanical surface treatments (msts) and two bonding systems. materials and methods: eighty-four sscs were divided into six groups of 14; group1: no mst+scotchbond universal adhesive (n+u), group 2: surface roughening by a diamond bur+scotchbond universal adhesive (r+u), ...

We have modified the automated differential display reverse transcription polymerase chain reaction technique (DDRT-PCR) such that a single fluorescently labeled universal primer (d(F)CTCACG-GATCCGTCGATTTT) is used in all PCRs together with a selection of arbitrary primers. We term this fluorescent detection procedure FDDRT-PCR. Anchoring primers of general structure dTGGTCTCACGGATCCTCGA-(T)12 ...

In the coming years, the frequency of unauthorised genetically modified organisms (GMOs) being present in the European food and feed chain will increase significantly. Therefore, we have developed a strategy to identify unauthorised GMOs containing a pCAMBIA family vector, frequently present in transgenic plants. This integrated approach is performed in two successive steps on Bt rice grains. F...