PURPOSE To evaluate different protocols to isolate stem cells from ovine umbilical cord blood and adipose tissue. METHODS There were used 5 samples of umbilical blood and 5 samples of perirenal adipose tissue from 10 female sheep. All the samples were obtained through surgery, to harvest aseptic samples. There were used 3 protocols for obtainment and culture of umbilical cord blood stem cells...

s:3604:"During pregnancy, the placenta delivers "cord blood" to the baby through the umbilical cord serving as a lifeline of nourishment from the mother to baby. At birth, "cord blood" remains in the umbilical cord and placenta and until recently, had typically been discarded. The tragedy of this practice is that "cord blood" contains very special cells called "stem cells". Recent advances in m...

Umbilical cord blood is the baby's blood left over in the umbilical cord and placenta after a baby is delivered and the cord is cut. Cord blood has all of the features of regular blood, but it is also a rich source of hematopoietic stem cells. Hematopoietic stem cells are unspecialized cells that produce all blood cells. These include: • Platelets, which are needed for blood clotting • Red...

Cord blood is the blood that remains in the umbilical cord and placenta following birth, which is usually discarded It contains red blood cells, white blood cells, platelets, and plasma, like blood. In addition, cord blood is a rich source of stem cells that may have potentially lifesaving benefits for your baby and family. The cord blood of baby serves as an abundant source of stem cells. Thes...

Ex vivo expansion of human umbilical cord blood cells (HUCBC) is explored by several investigators to enhance the repopulating potential of HUCBC. The proliferation and expansion of human hematopoietic stem cells (HSC) in ex vivo culture was examined with the goal of generating a suitable clinical protocol for expanding HSC for patient transplantation. Using primary human mesenchymal stem ...

Cord blood hematopoietic stem cells are widely used as an alternative source for hematopoietic stem cells transplant. Increasing rate of patients who need hematopoietic stem cells transplant and many advantages of cord blood in comparison to bone marrow hematopoietic stem cells, have promoted banking of cord blood units. Cord blood banking requires accurate steps in donor selection, cord blood ...

............................................................................................................ 8 REVIEW OF THE LITERATURE .......................................................................... 9 1. UMBILICAL CORD BLOOD STEM CELLS .................................................. 9 1.1 Umbilical cord blood as a stem cell source .......................................... 9 1.2 ...

background: stem cell therapy for the treatment of vascular-related diseases through functional revascularization is one of the most important research areas in tissue engineering. the aim of this study was to investigate the in vitro differentiation of umbilical cl-msc into endothelial lineage cells. methods: in this study, isolated cells were characterized for expression of msc-specific marke...