BACKGROUND Chinese medicinal herbs may use more than one species of Curcumae Radix (Yujin) is the tuberous roots of Curcumae wenyujin, C. kwangsiensis, C. phaeocaulis and C. longa. This study aimed to characterize the chemical profiles of these different species of Curcumae Radix, and develop a method for rapid discrimination of these species by ultra-high performance liquid chromatography-quad...

To determine the monoamines dopamine (DA), noradrenaline (NAD) and serotonin (5-HT) in microdialysis samples, ion-pair liquid chromatography (LC) with electrochemical detection (ECD) is typically applied. Because of the low concentrations in the extracellular space (pM range), further dilution in the microdialysis samples, the complexity of the samples and the required temporal resolution, the ...

BACKGROUND Murraya exotica L., a member of family Rutaceae is rich in biologically active secondary metabolites and also known for its ethnobotanical importance. With this in mind, the plant was chosen and leaves were extracted sequentially to obtain ethanol, hexane, chloroform, ethyl acetate, n-butanol and aqueous fractions. The extract/fractions were evaluated for total phenolic and flavonoid...

Two analytical platforms, gas chromatography (GC) coupled to quadrupole-time-of-flight (QTOF) mass spectrometry (MS) and reversed-phase ultrahigh performance liquid chromatography (UHPLC) coupled to diode array (DAD) and QTOF detection, were applied in order to study the alkaloid profile of Fumaria capreolata. The use of these mass analyzers enabled tentatively identifying the alkaloids by matc...

Traditional Chinese medicine (TCM) has been used for the treatment of many complex diseases. However, the bioactive components are always undefined. In this study, a bioactivity-based method was developed and validated to screen lipase inhibitors and evaluate the effects of processing on the lipase inhibitory activity of TCM by ultrahigh performance liquid chromatography coupled with quadrupole...

PSORI-CM01 is a Chinese medicine formula prepared from medicinal herbs and used in China for the treatment of psoriasis. However, the chemical constituents in PSORI-CM01 have not been clarified yet. In order to quickly define the chemical profiles and control the quality of PSORI-CM01 preparations, ultra-high liquid chromatography coupled with electrospray ionization hybrid linear trap quadrupo...

Conventional strategies for the screening of DNA triplex binders cannot be used for complicated samples, such as ligand libraries created by combinatorial chemistry or from natural product extracts. In the current study, an ultra-high-performance liquid chromatography coupled with an Orbitrap mass spectrometry (UHPLC-Orbitrap-MS)-based approach, which we call peak area-fading (PAF) UHPLC-Orbitr...

The present contribution discusses recent advances in ultra-high-pressure liquid chromatography (UHPLC) and multi-dimensional (MDLC) technology. First, new developments UHPLC column technology system design are highlighted. latter includes a description of novel injector concept enabling method speed-up, emerging detectors, instrument diagnostics approaches. Next, online MDLC workflows reviewed...

DNA and RNA chromatography is extensively used for nucleic acid analysis. To better understand the chromatographic mechanisms by which DNA and RNA oligonucleotides are separated, ion pair reverse-pair ultra-high performance liquid chromatography (IP RP UHPLC) methods were developed. 11mer and 12mer DNA and RNA oligonucleotides of various compositions were used during this study. The first part ...

Yak milk is an essential and predominant food resource for Tibetan people subsistence purposes to combat altitude-induced challenges. Due its unique qualities, yak has recently been gaining broader attention from consumers across China as well in other parts of the world. One key characteristics protein content, which about 40 60% higher than that native bovine milk. In this work, a sensitive r...