Endogenous trypsin inhibitors are synthesized, stored, and secreted by pancreatic acinar cells. It is believed that they play a protective role in the pancreas by inhibiting trypsin within the cell should trypsinogen become prematurely activated. Rodent trypsin inhibitors are highly homologous to human serine protease inhibitor Kazal-type 1 (SPINK1). The mouse has one pancreatic trypsin inhibit...

Several plant organs contain proteinase inhibitors, which are produced during normal plant development or are induced upon pathogen attack to suppress the enzymatic activity of phytopathogenic microorganisms. In this study, we examined the presence of proteinase inhibitors, specifically trypsin inhibitors, in the leaf extract of Capsicum baccatum var. pendulum inoculated with PepYMV (Pepper yel...

The evolutionary success of phytophagous insects depends on their ability to efficiently exploit plants as a source energy for survival. Herbivorous largely depend the efficiency, flexibility, and diversity digestive physiology sophistication detoxification system use chemically diverse host food sources. fall armyworm, Spodoptera frugiperda (J.E. Smith), is polyphagous pest many commercially i...

Trypsin inhibitor DE-3 from Erythrina latissima seeds contains 172 amino acids, including 4 half-cystine residues, and resembles the Kunitz-type inhibitors. Limited hydrolysis of DE-3 with trypsin at pH 3 produced two fragments, F1 and F2, containing 63 and 109 amino acids, respectively. Amino-terminal sequence studies revealed that F1 was the N-terminal and that F2 was the C-terminal fragment....

The use of hempseed flours in the animal diets may be limited by the presence of antinutritive compounds. The content of phytic acid, condensed tannins, trypsin inhibitors, cyanogenic glycosides and saponins was evaluated in hempseed meal of three italian varieties (dioecious) and three french varieties (monoecious) of Cannabis sativa L. The analysis of variance showed significant differences a...

Soybean (Glycine max (L.) Merr.) has emerged as one of the most economical and nutritious foods. Kunitz Soybean Trypsin Inhibitor (KSTI) and Bowman -Birk Inhibitor (BBI) are the two major trypsin inhibitors in soybeans. The ingested soybean trypsin inhibitors cause growth depression as demonstrated in different animal species and human. The main part of the present study was devoted to isolatio...

The production of protease activity by the sugarbeet pathogen Aphanomyces cochlioides, the legume pathogen A. euteiches, and the fish pathogen Saprolegnia parasitica was examined. Protease activity was readily detected in supernatants of water cultures of each organism using autoclaved host tissue as a nutrient source. Most of the protease isozymes extracted from sugarbeet and pea seedlings inf...

The germination of Bacillus cereus T spore suspensions is partially prevented by several inhibitors of trypsin-like enzymes. Leupeptin, antipain, and tosyl-lysine-chloromethyl ketone are effective inhibitors, whereas chymostatin, elastatinal, and pepstatin are inactive. A synthetic substrate of trypsin, tosyl-arginine-methyl ester, also inhibits germination. Its inhibitory effect decreases as a...