Virus-induced gene silencing has been suggested as a powerful and rapid technique for analysis of gene function in plant growth and development (Baulcombe, 1999). Infecting Nicotiana benthamiana with Tobacco Rattle Virus (TRV) containing a fragment of the gene encoding phytoene desaturase, an essential enzyme in carotene synthesis, results in a photo-bleached phenotype in photosynthetic tissues...

Recently, pathogenesis studies, using genetically distinct turkey-origin reoviruses (TRVs), revealed that poults infected with certain TRV isolates had moderate to severe bursal atrophy, suggesting virus-induced immune dysfunction. In order to characterize the effect of TRV infection on the turkey immune system, classical assays were undertaken to quantify the humoral and cell-mediated immune r...

The RNAi model predicts that during antiviral defense a RNA-induced silencing complex (RISC) is programmed with viral short-interfering RNAs (siRNAs) to target the cognate viral RNA for degradation. We show that infection of Nicotiana benthamiana with Tobacco rattle virus (TRV) activates an antiviral nuclease that specifically cleaves TRV RNA in vitro. In agreement with known RISC properties, t...

PURPOSE To determine the effect of optical defocus on the test-retest variability (TRV) of visual acuity measurements in normal subjects. METHODS Normal subjects underwent repeated visual acuity measurement with optical defocus of 0, 0.50, and 1.00 D. All measurements were taken using the Early Treatment Diabetic Retinopathy Study (ETDRS) version of the Bailey-Lovie logMAR chart. TRV was quan...

Due to their capability of eliciting a form of posttranscriptional gene silencing (termed virus-induced gene silencing or VIGS), plant viruses are increasingly used as reverse-genetics tools for functional characterization of plant genes. RNA viruses have been shown to trigger silencing in a variety of host plants, including members of Solanacae and Arabidopsis (Arabidopsis thaliana). Several f...

Using a series arrangement of two vacuum interrupters (VIs) has been proved to be an effective way of developing higher voltage vacuum circuit breakers (VCBs), which are known as double-break VCBs. The uniform distribution of the transient recovery voltage (TRV) across each VI is very critical for the breaking capacity of double-break VCBs. In this paper, the TRV distribution characteristics of...

Dicistroviridae is a new family of small, nonenveloped, and +ssRNA viruses pathogenic to both beneficial arthropods and insect pests as well. Triatoma virus (TrV), a dicistrovirus, is a pathogen of Triatoma infestans (Hemiptera: Reduviidae), one of the main vectors of Chagas disease. In this work, we report a single-step method to identify TrV, a dicistrovirus, isolated from fecal samples of tr...

Three possibilities for the origin of CP violation are discussed: (1) the Standard Model in which all CP violation is due to one parameter in the CKM matrix, (2) the superweak model in which all CP violation is due to new physics and (3) the Standard Model plus new physics. A major goal of B physics is to distinguish these possibilities. CP violation implies time reversal violation (TRV) but di...

Tobacco mesophyll protoplasts infected with raspberry ringspot virus (RRV) show faint generalized staining when treated with fluorescent antibody to RRV, but many brightly staining granules develop after inoculation with a mixture of RRV and the CAM strain of tobacco rattle virus (TRV-CAM). The phenomenon occurred with three different strains of RRV but not when tobacco mosaic virus, or two oth...

A reliable method to eliminate tobacco rattle virus (TRV) from viruliferous Paratrichodorus allius populations was developed. This virus is vectored by P. allius in the Pacific Northwest and causes corky ringspot disease (CRS) of potato. The viruliferous nematodes that were reared on 'Vernema' alfalfa or '770' scotch spearmint for at least 3 months did not transmit TRV to 'Samsun NN' tobacco, a...