Phenotypically, Photobacterium damselae subsp. piscicida and P. damselae subsp. damselae are easily distinguished. However, their 16S rRNA gene sequences are identical, and attempts to discriminate these two subspecies by molecular tools are hampered by their high level of DNA-DNA similarity. The 16S-23S rRNA internal transcribed spacers (ITS) were sequenced in two strains of Photobacterium dam...

PreQ1 (2) is a precursor of queuine (1) that in eubacteria incorporated into transfer RNA (tRNA) by tRNA guanine transglycosylase (TGT) before being further elaborated queuine. The modification unusual and occurs across all eukaryotes with few exceptions, but its function remains unclear. As the modified nucleotide through incorporation specially synthesized instead via genetically encoded base...

The Coregonus lavaretus (Linnaeus, 1758) complex is a morphologically and genetically diverse group of whitefish. Its taxonomic structure has been controversial for almost century. At least 25 forms C. have described in Siberia, but there still no consensus on their intraspecific taxonomy. pidschian (Gmelin, 1789) was as subspecies lavaretus. Recently, it assumed that this also complex. purpose...

We sampled 119 Nelore cattle (Bos indicus), 69 harboring B. indicus mtDNA plus 50 carrying Bos taurus mtDNA, to estimate the frequencies of putative mtDNA single nucleotide polymorphisms (SNPs) and investigate their association with Nelore weight and scrotal circumference estimated breeding values (EBVs). The PCR restriction fragment length polymorphism (PCR-RFLP) method was used to detect poly...

Transfer RNAs isolated from E. coli grown in media where ferric iron is not freely available show well characterized chromatographic changes due to the absence of the methylthio moiety of ms2i6A. The altered tRNA molecules include tRNA trp tRNA tyr, tRNA phe and two minor tRNA ser species. It has been suggested that methylthiolation of tRNA affects its function in regulation. We now show iron-r...

Formyltetrahydrofolic acid:methionyl-tRNA transformylase was isolated from Saccharomyces cerevisiae mitochondria and used to prepare yeast mitochondrial [(3)H]formylmethionyl-tRNA. This fMet-tRNA hybridizes with mitochondrial DNA but not with yeast nuclear or E. coli DNA. Unlabeled mitochondrial, but not extramitochondrial, tRNA competes in this reaction. tRNA was eluted from the hybrid and fou...

Leafhoppers have been identified as a serious threat to different plants. To explore the characteristics of mitogenomes and reveal phylogenetic positions two species in Typhlocybinae, complete Singapora shinshana Seriana bacilla were sequenced annotated for first time with lengths 15,402 bp 15,383 bp, respectively. The contained 13 PCGs, 22 tRNA genes 2 rRNA genes. genome content, gene order, n...

In most prokaryotes Asn-tRNA and Gln-tRNA are formed by amidation of aspartate and glutamate mischarged onto tRNA and tRNA, respectively. Coexistence in the organism of mischarged Asp-tRNA and Glu-tRNA and the homologous Asn-tRNA and Gln-tRNA does not, however, lead to erroneous incorporation of Asp and Glu into proteins, since EF-Tu discriminates the misacylated tRNAs from the correctly charge...

The recognition of human tRNA(Leu) or tRNA(Ser) by cognate aminoacyl- tRNA synthetases has distinct requirements. Only one base change (A73-->G) in tRNA(Leu) is required to generate an efficient serine acceptor in vitro, whereas several changes in three structural domains (the acceptor stem, DHU loop and long extra arm) of tRNA(Ser) are necessary in order to produce a leucine acceptor. Hence, t...