Complex I11 (ubiquinol-cytochrome c reductase) was purified from beef heart mitochondria in the form of protein-phospholipid-Triton X100 mixed micelles (about 1:SO:lOO molar ratio). Detergent may be totally removed by sucrose density gradient centrifugation, and the resulting lipoprotein complexes retain full enzyme activity. In order to understand the role of surfactant in the mixed micelles, ...

Band 3 (MI = 95,000), the anion exchange protein of human erytbrocyte membranes, is a transmembrane glycoprotein that is a dimer in the membrane and in Triton X-100 solutions. Mild trypsin treatment of erythrocyte ghosts cleaves Band 3 at the inner membrane surface producing a water-soluble fragment (& = 41,000) and a membrane-associated fragment (Mr = 55,000). The 41,000-dalton fragment, which...

AlkL is an outer membrane porin from Pseudomonas. Its expression in E. coli was shown to greatly stimulate the oxidation of hydrophobic substrates by a a recombinant oxygenase enzyme system. Bacterial membranes can be perturbed for biotechnological purposes by enzymatic treatment. Some of the enzymes and their actions are discussed on this commercial site. Different chemical treatments that are...

A direct method is presented for the determination of manganese in whole blood or serum by atomic absorption spectroscopy, using a graphite furnace with a temperature ramp controller. Blood or serum is diluted with an equal volume of a five percent solution of Triton-X100 containing heparin. This prevents the blood from coagulating and produces a solution with a viscosity suitable for injection...

Phosphatidylglycerophosphate synthase (sn-glycerol-3-phosphate:CDP-diacylglycerol phosphatidyltransferase) and phosphatidylglycerophosphate phosphatase were characterized in mung bean (Vigna radiafa 1.) mitochondria. The synthase has a rather broad pH optimum between 7 and 9, whereas the phosphatase has one of about 7. 60th enzymic activities are stimulated by Triton X100 and require divalent c...

INTRODUCTION: The use of injectable scaffolds has raised great interest as they minimise the need for invasive surgery and its associated complications, costs and discomfort to the patient. Furthermore, they can fill cavities of any size or shape as well as being able to deliver a localised therapeutic agent [1]. The aim of this study was to develop an injectable scaffold using PLGA micropartic...

It has been suggested that proteins act as primers for the synthesis of polysaccharides in general [ 11 and for plant-cellwall polysaccharides in particular 121. These primer proteins might anchor nascent matrix polysxcharides to the membrane during their passage through the endomembrane system from the Golgi apparatus to the cell wall 131. In this paper we give e v i d e n c e f o r a p r o t ...

Two populations of nerve growth factor (NGF) receptors can be distinguished on PC12 cells and have been termed “fast” and “slow” receptors on the basis of their respective rates of dissociation. Since a slowly dissociating state of the NGF receptor could be produced by receptor clustering, we sought to examine the effects of artificially clustering NGF-receptor complexes by using anti-NGF antib...