The development of a method based on extraction induced by emulsion breaking (EIEB) aiming copper determination in gasohol using graphite furnace atomic absorption spectrometry (GF AAS) is reported. Emulsification the samples was carried out nitric acid solution, (1,1,3,3-tetramethylbutyl)phenyl-polyethylene glycol surfactant (Triton X-114), and ultrasound energy. variables related to efficienc...

A method based on cloud point extraction was developed to determine phthalate esters including di-ethyl-phthalate (DEP), di-(2-ethylhexyl)-phthalate (DEHP) and di-cyclohexyl-phthalate (DCP) in environmental water samples using high-performance liquid chromatography separation and ultraviolet detection (HPLC-UV). The non-ionic surfactant Triton X-114 was chosen as extraction solvent. The paramet...

  Abstract A simple, sensitive and feasible cloud-point extraction (CPE) methodology has been developed for the separation and preconcentration of cadmium and lead ions in real samples. The metals in the aqueous solution were complexes with 2-(3- indolyl) - 4,5 di phynyl imidazole.(IDPI), at pH = 7.0 and Triton X-114 was added as surfactant. The variables effecting like concentration of Triton ...

Integral membrane proteins that form water-filled channels through membranes often exist as aggregates of similar or identical subunits spanning the membrane. It has been suggested that the insertion into the membrane of the channel-forming domains of the subunits may impart unusual structural features to the membrane-intercalated portions of the protein. To test this proposal, we have investig...

Temperature-induced phase separation in Triton X-114 (Bordier, C. (1981) J. Biol. Chem. 256, 1604-1607) and charge-shift electrophoresis (Helenius, A., and Simons, K. (1977) Proc. Natl. Acad. Sci. U. S. A. 74, 529-532) were used to examine the amphiphilic character of hormone-sensitive lipase, purified from rat adipose tissue. In contrast to ATP-citrate lyase, a reference hydrophilic protein, t...

Alphalactalbumin (α-La) and betalactoglobulin (β-Lg) in the rehydration of bovine colostrum powder were successfully separated by cloud point extraction using a nonionic surfactant Triton X-114. The effects of different factors, including the surfactant concentration, sample volume, electrolyte, and pH were discussed. The optimized conditions for cloud point extraction of alphalactalbumin (α-La...

Triton X-114 (Triton X-114) surfactant separates into two isotropic phases at room temperature and can be successfully used in cloud point extraction for the analysis of polycyclic aromatic hydrocarbons (PAHs). We studied which type of container is the most suitable for PAHs extraction with this method and how the water affects PAH recovery. We used a generator and mini-centrifuge with a cloud ...

Polyphenoloxidase from grape berries is extracted only by nonionic detergents with a hydrophilic-lipophilic balance between 12.4 and 13.5. The enzyme was partially purified in latent form, free of phenolics and chlorophylls, by using temperature phase partitioning in a solution of Triton X-114. This method permits the purification of the enzyme with the same fold purification as the commonly us...