cotton (gossypium hirsutum l.)  is an important fiber crop in iran, cultivated on 150000-200000 ha of land.  in iran the estimated loss due to the insect pest is more than 30%. traditionally, pests are controlled by 10-12 times spraying per growing season of environmentally harmful chemical insecticides (e.g. endosulfan and/or methosystox). in order to produce transgenic cotton resistance to in...

abstract background: selectable marker gene (smg) systems are critical for generation of transgenic crops. transgenic crop production background: selectable marker gene (smg) systems are critical for generation of transgenic crops. transgenic crop production without using smg is not economically feasible. however, smgs are non-essential once an intact transgenic plant has been established. elim...

Background: In the last two decades, genetic engineering has led to the production of genetically modified or transgenic products in the world using the modern biotechnology. However, accepting such products by consumers is still in doubt with respect to their negative effects on the environment and human health. Thus, the present study aimed to evaluate the attitude of housewives in Yazd towar...

somatic embryos of persian walnut were transformed with two strains of agrobacterium tumefaciens i.e. lba4404 and c58, and two plasmids, namely, pbi121 with nptii and gus genes for improving the transformation protocol, and p6u-ubi-fvti plasmid containing the hpt and fld genes. the transformation frequency was 10%. pcr and rt-pcr analysis proved the presence and expression of the genes. the tra...

a significant portion of the world’s cultivated land is affected by salinity that reduces crop productivity in these areas. breeding for salt tolerance is one of the important strategies to overcome this problem. recently, genetic engineering is becoming a promising approach to improving salt tolerance. in order to improve the yield performance of canola in saline soils, we transformed canola w...

in this study proline content and activity of catalase (cat), and ascorbate peroxidase (apx) and level of lipid peroxidation in terms of malondialdehyde (mda) content were measured in transgenic tobacco (nicotiana tabacum cv. wisconsin), over expressing a δ-1-pyrroline-5-carboxylate synthase (p5cs) gene, and non transgenic plants as control. drought stress was applied using polyethylene glycol ...

production of transgenic cotton (gossypium hirsutum l.) was studied utilizing agrobacterium-mediated strain lba4404 and pbi121 plasmid. this plasmid contains gus as a reporter gene driven by the camv35s promoter and nptii gene driven by nos as selection marker. in this study, the inheritance of gus gene was evaluated using selective media, pcr and gus staining in the second genertaion (t1) of t...

Cotton (Gossypium hirsutum L.)  is an important fiber crop in Iran, cultivated on 150000-200000 ha of land.  In Iran the estimated loss due to the insect pest is more than 30%. Traditionally, pests are controlled by 10-12 times spraying per growing season of environmentally harmful chemical insecticides (e.g. endosulfan and/or methosystox). In order to produce transgenic cotton resistance to in...

To investigate the impact of overexpression of AtNRT2.1 transgene from Arabidopsis on nitrate uptake rate and to understand the regulation of endogenous HATS by nitrate and glutamine amino acid (Gln) in tobacco plants, wild-type and transgenic (F line) plants grown on soil for 4 weeks were transferred to hydroponic culture in a controlled-environment with a 16/8h L:D photoperiod at 24? C/20...