UNLABELLED BACKGROUND Industrial production of biofuels and other products by cellulolytic microorganisms is of interest but hindered by the nascent state of genetic tools. Although a genetic system for Clostridium thermocellum DSM1313 has recently been developed, available methods achieve relatively low efficiency and similar plasmids can transform C. thermocellum at dramatically different ...

genetic transformation studies were carried out to standardize a protocol for agrobacterium -mediated genetic transformation of three economically important strawberry (fragaria x ananassa duch) cultivars kurdistan’, camarosa’, and ‘paros’. shoot regeneration frequency 72, 65 and 30% was obtained on ms (1) basal medium supplemented with 2% glucose and 4 mg/l tdz for camarosa, kurdistan and paro...

Agrobacterium tumefaciens mediated transformation may offer a better alternative than the biolistic gun for genetic transformation of maize plants. This gene delivery system results in a greater proportion of stable, low-copy number transgenic events than does the biolistic gun, and is highly efficient. In the present work, we studied maize transformation using A. tumefaciens by identifying som...

The effects of interactions between genetic materials and polycyclic aromatic hydrocarbons (PAHs) on gene expression in the extracellular environment remain to be elucidated and little information is currently available on the effect of ionic strength on the transformation of plasmid DNA exposed to PAHs. Phenanthrene and pyrene were used as representative PAHs to evaluate the transformation of ...

A design of a compact, easy to fabricate and applicable structure rectangular waveguide TM11 to TE10 mode converter is presented in this paper. The design procedure of the proposed structure can be divided into two parts in sequence. The beginning one is dedicated to the transformation from TM11 to TEM mode using a central conductor, while the second part is the transformation from TEM to TE10 ...