Aflatoxins B! and G,, in single doses, were lethal and had similar relative potencies in the duckling (50% lethal dose, 0.73 mg/kg versus 1.18 mg/kg) and in the rat (50% lethal dose, 1.16 mg/kg versus 2 to 4 mg/kg). Aflatoxins B2 and G2 were less potent in the duckling (50% lethal dose, 1.76 mg/kg versus 2.83 mg/kg) and were nontoxic to rats at doses of 200 mg/kg. Aflatoxin BI induced hepatocel...

OBJECTIVES Investigations were carried out to determine aflatoxin levels in household maize in Makueni District and to correlate aflatoxin levels to maize drying and storage practices. Also, aflatoxin exposure in villages that reported aflatoxicosis cases in 2005 was compared with that in villages that did not report cases to assess whether aflatoxin exposure levels could be used to identify hi...

The aim of this study was to evaluate the efficacy of a yeast glucomannan (YG) and sodium bentonite (SB) in reducing the toxicity of aflatoxin for broilers fed a diet naturally contaminated with aflatoxin. In total, 300 7-day-old Ross 308 broilers were randomly assigned to 10 dietary treatments, consisting of a diet with no aflatoxin (positive control), a diet naturally contaminated with 250 pp...

The metabolism of aflatoxin B, has been investigated in male rhesus monkeys given i.p. injections of a mixture of ring-labeled aflatoxin B!-'4C and nonradioactive toxin, at a total dose of 0.4 mg/kg. Thirty-five % of the administered radioactivity was excreted in urine within 96 hr. The excretion rate reached a maximum 1 hr after dosing and then fell rapidly, but urine excreted after 96 hr stil...

This paper reports the evidence for the existence of multiple sites of action of aflatoxin B, in relation to its inhibition of rat hepatic nuclear RNA synthesis. Two hours after aflatoxin B, injection (0.3 mg/lOO g body weight), rat hepatic nuclear and nucleolar RNA synthesis, in vitro, were inhibited 70 and 90% respectively. When total nuclear free and engaged RNA polymerases were solubilized ...

This paper reports the evidence for the existence of multiple sites of action of aflatoxin B1 in relation to its inhibition of rat hepatic nuclear RNA synthesis. Two hours after aflatoxin B1 injection (0.3 mg/100 g body weight), rat hepatic nuclear and nucleolar RNA synthesis, in vitro, were inhibited 70 and 90% respectively. When total nuclear free and engaged RNA polymerases were solubilized ...

To search for an alternative to using protein conjugated aflatoxin as a coating antigen in aflatoxin detection by an ELISA method, a random-8-peptide library was constructed and used as a source of peptides that mimic aflatoxins (termed as mimotopes). Five mimotope peptides were obtained by panning-elution from the library and were successfully used in an indirect competitive ELISA for analyzin...

This paper reports the evidence for the existence of multiple sites of action of aflatoxin B, in relation to its inhibition of rat hepatic nuclear RNA synthesis. Two hours after aflatoxin B, injection (0.3 mg/lOO g body weight), rat hepatic nuclear and nucleolar RNA synthesis, in vitro, were inhibited 70 and 90% respectively. When total nuclear free and engaged RNA polymerases were solubilized ...

In this paper, the effect of infrared (IR) roasting conditions on Aflatoxins and total counts of two types of Iranian pistachio kernels, long and round, was studied. The optimized roasting conditions, 70 V for round pistachios and 90 V for long pistachios with 10 cm distance from IR source were used. Naturally Aflatoxin-contaminated kernels were supplied and roasted. Microbiological an...

In an earlier report, it was shown that aflatoxin B1 treatment strongly inhibits rat liver nucleolar RNA synthesis (Yu, F. L. (1977) J. Biol. Chem. 252, 3245-3251). The present paper is an attempt to elucidate the mechanism of this inhibition. Two h after aflatoxin B1 injection (0.3 mg/100 g body weight), rat liver nucleolar RNA synthesis, in vitro, was inhibited by an average of 90%. This inhi...