Electron microscopy and X-ray scattering show that double-stranded DNA is wound in a regular way in the heads of phage such as p22 and ).. DNA is "pumped" into such heads, which may simply act as passive containers. In contrast, the protein-coat subunits of tomato bushy stunt virus have a flexibly tethered N-terminal domain that appears to be a major locus of interactions with the compactly fol...

The genomes of plus-strand RNA viruses contain many regulatory sequences and structures that direct different viral processes. The traditional view of these RNA elements are as local structures present in non-coding regions. However, this view is changing due to the discovery of regulatory elements in coding regions and functional long-range intra-genomic base pairing interactions. The ∼4.8 kb ...

Tomato bushy stunt virus (TBSV) is one of few RNA plant viruses capable of moving systemically in some hosts in the absence of coat protein (CP). TBSV also encodes another protein (p19) that is not required for systemic movement but functions as a symptom determinant in Nicotiana benthamiana. Here, the role of both CP and p19 in the systemic spread has been reevaluated by utilizing transgenic N...

The structure of recombinant virus-like particles of malabaricus grouper nervous necrosis virus (MGNNV), a fish nodavirus isolated from the grouper Epinephelus malabaricus, was determined by electron cryomicroscopy (cryoEM) and three-dimensional reconstruction at 23-A resolution. The cryoEM structure, sequence comparison, and protein fold recognition analysis indicate that the coat protein of M...

The electrophoretic patterns of dsRNA species isolated from cucumber plants infected with cucumber necrosis virus (CNV) and from Nicotiana clevelandii plants infected with tomato bushy stunt virus (TBSV) were almost identical and differed greatly from the electrophoretic patterns of dsRNA species isolated from tobacco plants infected with tobacco necrosis virus (TNV). Hybridization with cloned ...