The purpose of this in vitro study was to evaluate the antimicrobial effect of photodynamic therapy on Streptococcus mutans (A TCC 25175) suspensions, using a red laser for one minute in combination with toluidine blue O (TBO) or methylene blue (MB). Both photosensitizers were used in three concentrations (25, 10 and 5 mg/L). The activity ofphotosensitizers and laser irradiation were tested sep...

There is increasing evidence that the redox activities of the pulmonary endothelial surface may have important implications for the function of both lungs and blood. Because of the inherent complexity of intact organs, it can be difficult to study these activities in situ. Given the availability of appropriate indicator probes, the multiple-indicator dilution (MID) method is one approach for de...

1. Photodynamic therapy could be an alternative treatment for highly prevalent local antibiotic-resistant pathogens. 2. Photodynamic inactivation using toluidine blue O was observed on both reference strains and clinical samples, including methicillin-resistant Staphylococcus aureus, e x t e n d e d s p e c t r u m β l a c t a m a s e – p r o d u c i n g Escherichia coli and Klebsiella pneumoni...

Purpose: The objects of this study were to compare biological properties of a native articular cartilage extracellular matrix (ACECM)-derived oriented scaffold and the Chondro-Gide bilayered scaffold. Methods: The ACECM oriented scaffold (No. 4131) and the Chondro-Gide scaffold (No. 4132) compared form (I) The physical properties (II) FDA/Pl staining, and frozen sections were stained with tolui...

BACKGROUND Trichophyton rubrum is the most common agent of superficial mycosis of the skin and nails causing long lasting infections and high recurrence rates. Current treatment drawbacks involve topical medications not being able to reach the nail bed at therapeutic concentrations, systemic antifungal drugs failing to eradicate the fungus before the nails are renewed, severe side effects and s...

OBJECTIVE The aim of this study was to assess the importance of early diagnosis of potentially malignant lesions in the prevention of oral cancer. MATERIAL AND METHODS In order to achieve the set objectives, we selected our participant pool. Of the 340 patients who came to the initial examination at the Clinic for Oral Medicine and Periodontology, we selected 40 patients with potentially mali...

Malaria, a febrile illness mostly confined to the tropical countries is transmitted by bite of infected female Anopheles mosquito. In 2015 alone, 88% of the malaria burden and 90% deaths due to malaria were confined to the African and Asian countries. Although number of tests are available for rapid diagnosis and screening for malaria, peripheral blood smear examination remains the gold standar...

Studies were carried out on the photodynamic inactivation of Bacillus subtilis cells by He-Ne laser irradiation in presence of methylene blue and toluidine blue. Electron paramagnetic resonance (EPR) measurements show decrease in the cell membrane nuidity due to photodynamic damage of the lipophilic compartments. Study of cell damage in presence of sodium azide, 0 20 and glutathione as well as ...

Our improved toluidine blue-DNA agar was compared with methyl green DNase test agar for the detection of DNA hydrolysis by campylobacters. The toluidine blue-DNA agar gave clear-cut positive and negative reactions with campylobacter strains belonging to several species.