نتایج جستجو برای: tissue plasminogen activator t
تعداد نتایج: 1581327 فیلتر نتایج به سال:
Confluent cultures of endothelial cells from human umbilical cord were used to study the effect of activated human protein C (APC) on the production of plasminogen activators, plasminogen activator-inhibitor, and factor VIII-related antigen. Addition of APC to the cells in a serum-free medium did not affect the production of tissue-type plasminogen activator (t-PA) or factor VIII-related antige...
Low molecular size additives such as L-arginine and the redox compounds have been used both in the culturemedium and in vitro refolding to increase recombinant proteins production. Additives increase proteinrefolding and yield of active proteins by suppressing aggregate formation or enhancing refolding process.In this work, a comparative study was performed on refolding of rec...
Cultured human umbilical vein endothelial cells release tissue plasminogen activator ft-PA) and type 1 plasminogen activator inhibitor (PAl-i ) in response to alpha thrombin stimulation. In order to study the mechanisms of thrombin stimulation, we measured changes in levels of mRNA for t-PA and PAl-i following exposure of endothelial cells to 3 U/mI alpha thrombin. Alpha thrombin causes a signi...
The secretion of tissue plasminogen activator ft-PA). urokinase (u-PA) and their inhibitors by the human leukemia cell line K562 was examined. K562 cells normally secrete both t -PA and u-PA in a ratio of 3:1 . After addition of 10 or 1 ng/mL phorbol myristate acetate (PMA) to K562 cells. a marked decrease in enzymatic activity is observed in the medium. However. when t-PA antigen rather than a...
Two-chain tissue-type plasminogen activator (t-PA), which consists of a heavy chain (Mr congruent to 38,000) and a light chain (Mr congruent to 31,000) connected by a disulfide bridge, was reduced with 2-mercaptoethanol and then air-reoxidized at a low protein concentration and carboxamidomethylated. The two chains were separated by means of zinc chelate-agarose, which was found to bind the lig...
The neurovascular unit provides a dynamic interface between the circulation and central nervous system. Disruption of neurovascular integrity occurs in numerous brain pathologies including neurotrauma and ischaemic stroke. Tissue plasminogen activator is a serine protease that converts plasminogen to plasmin, a protease that dissolves blood clots. Besides its role in fibrinolysis, tissue plasmi...
1.1 The plasminogen activation system Initiation of the plasminogen activation system results in generation of the broad spectrum serine protease, plasmin, from the circulating zymogen, plasminogen. Plasminogen is activated to plasmin by plasminogen activators (PA’s), either urokinase type-plasminogen activator (u-PA) or tissue-type plasminogen activator (t-PA), via specific proteolytic cleavag...
The interaction of plasminogen, tissue plasminogen activator (t-PA) and urokinase with a clinical strain of Helicobacter pylori was studied. Plasminogen bound to the surface of H. pylori cells in a concentration-dependent manner and could be activated to the enzymatic form, plasmin, by t-PA. Affinity chromatography assays revealed a plasminogen-binding protein of 58.9 kDa in water extracts of s...
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