*Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada; Department of Large Animal Clinical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada; Center for Veterinary Drug Residues, Canadian Food Inspection Agency, Saskatoon Laboratory, Saskatoon, SK, Canada; Departm...

BACKGROUND The mitochondrial DNA (mtDNA) depletion syndromes (MDDSs) are autosomal recessive disorders characterized by a reduction in cellular mtDNA content. Mutations in at least 9 genes [POLG, polymerase (DNA directed), gamma; DGUOK, deoxyguanosine kinase; TK2, thymidine kinase, mitochondrial; TYMP, thymidine phosphorylase; MPV17, MpV17 mitochondrial inner membrane protein; SUCLA2, succinate...

Inflammatory disorders of the bowel and colon cancer are associated with elevated indices of oxidative stress. Analogous elevations in markers of oxidative stress and loss of cell-membrane integrity are also observed in the colons of rats deficient in vitamin E (D-alpha-tocopherol), the major lipid-soluble antioxidant in biological systems. The causal relationship between colon pathologies asso...

ayurveda, manoeuvres mankind to head a healthy life in order to pursue four-fold bliss. with a view to combat physical and mental annoyances, classics have explicated two-fold therapeutic modalities; langhana/depletion and brihmana/nourishing. upavasa /fasting is one among ten depletion therapies explained. it is envisioned at all three levels of ayurvedic therapies- rational/objectively planne...

OBJECTIVES To examine the in vivo effects of highly active antiretroviral therapy (HAART) regimens on adipose tissue mitochondrial DNA (mtDNA) depletion, mitochondrial organellar proliferation, and markers of adipocyte differentiation and phenotype. DESIGN AND METHODS DNA and mRNA quantification using real-time PCR methods was performed on adipose tissue samples from 31 HIV-infected individua...

The mechanisms linking human immunodeficiency virus replication to the progressive immunodeficiency of acquired immune deficiency syndrome are controversial, particularly the relative contribution of CD4+ T cell destruction. Here, we used the simian immunodeficiency virus (SIV) model to investigate the relationship between systemic CD4+ T cell dynamics and rapid disease progression. Of 18 rhesu...

The realization within recent years of the prevalence and seriousness of protein deficiency has emphasized the need for some simple means of recognizing the presenc e and degree of such a condition. The only proteins capable of direct clinical measurement are those in the blood. Yet there has, so far, been no evidence that a lowered plasma protein concentration reflects accurately anything but ...

 In Drosophila, some factors involved in chromosome replication seem to be involved in gene amplification and endoreplication, which are actively utilized in particular tissue development, but direct evidence has not been shown. Therefore, we examined the effect of depletion of replication factors on these processes. First, we confirmed RNAi knockdown can be used for the depletion of replicatio...