The Hunter (1) procedure was originally developed for the calorimetric d&&ion of minute amounts of thymine. In this procedure thymine is coupled under air with p-diazobenzenesulfonic acid in carbonate solut.ion, and a red color is subsequently produced by the addition of sodium hydroxide and hydroxylamine. Thymine was determined by this means in hydrolysates of dcsoxypentose nucleic acids (DNA)...

Thymine is the only nucleotide base which is changed to uracil upon transcription, leaving mRNA less hydrophobic compared to its DNA counterpart. All the 16 codons that contain uracil (or thymine in gene) as the second nucleotide code for the five large hydrophobic residues (LHRs), namely phenylalanine,v isoleucine, leucine, methionine and valine. Thymine content (i.e. the fraction of XTX codon...

Several thymineless Escherichia coli strains have been examined for their ability to replicate their deoxyribonucleic acid when bromouracil is substituted for thymine. The procedure we describe was used to identify a thymineless strain with characteristics relatively favorable to its use in bromouracil labeling experiments. In addition, mutants with an "absolute" thymine requirement could be ea...

Graphene-enhanced Raman scattering (GERS) spectra and coherent anti-Stokes Raman scattering (CARS) of thymine molecules adsorbed on a single-layer graphene were studied. The enhancement factor was shown to depend on the molecular groups of thymine. In the GERS spectra of thymine, the main bands are shifted with respect to those for molecules adsorbed on a glass surface, indicating charge transf...

Studies with Lactobadlus tichmannii have indicated that vitamin Blz is required for the reduction of formate to the methyl group of thymine (1). Such a function is in agreement with other data implicating the vitamin in methyl group neogenesis and in thymine synthesis (2, 3). The present experiments were designed to study the role of vitamin Bls in thymine biosynthesis by bone marrow. The resul...

Four double-headed nucleosides were prepared by the CuAAC reaction. Hereby, a triazole-containing linker connects an additional thymine or adenine to the 2'-position of 2'-deoxyuridine, a thymine to the 5'-position of thymidine and a thymine to the 6'-position of an LNA-thymidine monomer. Whereas no conclusive recognition effects of the additional thymines were found when introduced in LNA or a...

Thymidine phosphorylase (TP) catalyses the conversion of thymidine into thymine. A non-radiochemical assay procedure for TP was developed in which thymine was detected at 265 nm after separation with reversed-phase HPLC. A complete separation of thymidine and thymine was achieved in 6 min and the minimum amount of thymine that could be detected was 0.8 pmol. The assay was linear with reaction t...

To explore the excited-state structural dynamics of thymine, a DNA nucleobase, we measured the resonance Raman spectra of thymine in aqueous solution at wavelengths throughout the lowest-energy absorption band. Self-consistent analysis of the resulting resonance Raman excitation profiles and absorption spectrum using a time-dependent wave packet formalism yielded the excited-state structural dy...