Mahoney, Robert P. (Skidmore College, Saratoga Springs, N.Y.), and Mercedes R. Edwards. Fine structure of Thiobacillus thiooxidans. J. Bacteriol. 92: 487-495. 1966.-Thin section analysis of the chemosynthetic autotroph Thiobacillus thiooxidans revealed structures comparable to gram-negative heterotrophic bacteria. Although this species is unique in that it oxidizes elemental sulfur for energy, ...

introduction: sulfur is the key element for higher crops and plays an important role in the formation of proteins, vitamins, and enzymes. it is a constituent of amino acids such as cysteine and methionine, which act for the synthesis of other compounds containing reduced sulfur, such as chlorophyll and utilization of phosphorus and otheressential nutrients.deficiency of this nutrient in soil is...

Chemolithoautotrophic bacteria were enriched from mine water at incubation temperatures ranging from 4 to 46 degrees C, using elemental sulfur as a substrate in acid mineral salts media. Thiobacillus-type bacteria were successfully enriched for at all test temperatures except 46 degrees C. Changes in pH (-dpH/dt) were used to estimate the rate constants for the enrichment cultures. The rate con...

Compacted bentonites are one of the best sealing and backfilling clays considered for use in Deep Geological Repositories radioactive wastes. However, an in-depth understanding their behavior after placement repository is required, including if activity indigenous microorganisms affects safety conditions. Here we provide optimized phenol:chloroform based protocol that facilitates higher DNA-yie...

This study demonstrates the efficacy of a direct 5S rRNA assay for the characterization of mixed microbial populations by using as an example the bacteria associated with acidic mining environments. The direct 5S rRNA assay described herein represents a nonselective, direct molecular method for monitoring and characterizing the predominant, metabolically active members of a microbial population...

The effect of glutaraldehyde (GA) in different concentrations on microbiological activities of Bacillus licheniformis, Desulfotomaculum ruminis and Thiobacillus ferrooxidans bacteria used in the processes of denitrification, desulfurication and iron (II) oxidation, respectively, has been tested. For the sake of comparison, the effect of formaldehyde (FA) on the activities of the same organisms ...

The main intention of this study to understand the microbial consortium of Sulphate Reducing Bacteria(SRB) and Sulphure oxidizing bacteria (SOB) on Extra polymeric substance produced by Pseudomonas sp., with combination of E-coli. In stressing the importance of anaerobic corrosion by SRB, corrosive effects of acids produced by microorganisms may be overlooked Sulphur oxidizers chiefly of the ge...

The use of Thiobacillus ferrooxidans bacteria in the process of copper and iron leaching from chalcopyrite coming from Kotlina Ktodzka was investigated. The influence of the mineral content in the leaching solution the pH value and refinement of chalcopyrite on the subsequent stages of leaching was established. The process was described by the 1st order reaction inhibited by one of the products...

Thermothrix thiopara did not appear to be stressed at high temperature (72 degrees C). Both the actual and theoretical yields were higher than those of analogous mesophilic sulfur bacteria, and the specific growth rate (mu(max)) was more rapid than that of most autotrophs. The specific growth rate (0.58 h), specific maintenance rate (0.11 h), actual molar growth yield at mu(max) (Y(max) = 16 g ...

Seven strains of heterotrophic iron-oxidizing acidophilic bacteria were examined to determine their abilities to promote oxidative dissolution of pyrite (FeS2) when they were grown in pure cultures and in mixed cultures with sulfur-oxidizing Thiobacillus spp. Only one of the isolates (strain T-24) oxidized pyrite when it was grown in pyrite-basal salts medium. However, when pyrite-containing cu...