The cDNAs of lactate dehydrogenase b (LDH-b) from both deep-sea and shallow living fish species, Corphaenoides armatus and Gadus morhua respectively, have been isolated, sequenced and their encoded products overproduced as recombinant enzymes in E. coli. The proteins were characterised in terms of their kinetic and physical properties and their ability to withstand high pressures. Although the ...

Multicopper oxidases can act on a broad spectrum of phenolic and non-phenolic compounds. These enzymes include laccases, which are widely distributed in plants and fungi, and were more recently identified in bacteria. Here, we present the results of biochemical and mutational studies of small laccase (SLAC), a multicopper oxidase from Streptomyces coelicolor (SCO6712). In addition to typical la...

The human dimethylglycine dehydrogenase (hDMGDH) is a flavin adenine dinucleotide (FAD)- and tetrahydrofolate (THF)-dependent, mitochondrial matrix enzyme taking part in choline degradation, one-carbon metabolism and electron transfer to the respiratory chain. The rare natural variant H109R causes dimethylglycine dehydrogenase deficiency leading to increased blood and urinary dimethylglycine co...

kinetic parameters of the ammonolysis reaction of dichloroethane in nonaqueous media are studied and the optimum reaction conditions are reported. a suitable reactor for running the reaction under high pressure in the range of 50 to 500 °c is designed. kinetic behaviours of the system is studied and other reaction parameters such as rate constant, kinetic order, and activation energy are determ...

The effect of heat treatment on polyphenoloxidases and peroxidase activities in three tissues (cambium, central cylinder internal bark) the varietie cassava roots (Manihot esculenta CRANTZ) Bonoua 2 were studied over a range 30 to 70 °C, using pyrogallol, 4-methylcatechol, dopamine pyrocatechol as substrates for polyphenol oxydase guaiacol substrate peroxidase. Optimal conditions enzym...

We have earlier reported the synthesis and antisense properties of the conformationally constrained oxetane-C and -T containing oligonucleotides, which have shown effective down-regulation of the proto-oncogene c-myb mRNA in the K562 human leukemia cells. Here we report on the straightforward syntheses of the oxetane-A and oxetane-G nucleosides as well as their incorporations into antisense oli...

abstractthe purpose of this study was to investigate the controllable operating parameters influence, including ph, solid content, collector, co-collector, and depressant dose, and conditioning time, on apatite flotation kinetics. four first order flotation kinetic models are tested on batch flotation time-recovery profiles. the results of batch flotation tests and the fitting of first-order ki...

d Q-T interval have been reported Lancer 1998;352:162-3. 5 Waller PC, Coulson RA, Wood SM. Regulatory pharmacovigilance in the United Kingdom: current principles and practice. Pharnracoepidemwl Drug Safety 1996;5:363-75. 6 Li Wan Po A, Zhang WY. What lessons can be rk reiterates our learnt from withdrawal of mibefradil from the market? Lancer 1998;351:1829-30. with 7 Lawson DH. Pharmacovigilanc...

In this study simulation and optimization of an industrial acetylene hydrogenation reactor was studied. Three well known kinetic models were used for a nearly similar catalyst to predict the industrial data. Due to the complexity of the reactions, none of the offered kinetic models could be considered as an exact kinetic model and it is necessary to determine the kinetic parameters. One of the ...

The "differential" method of Achar, Brindley and Sharp (ABS) and the "integral" one of Coats and Redfern (CR) are applied to the dehydration of BaBr2-H20 and HCOOLiH20 and to the thermal decomposition of Pb(SCN)2, and the kinetic parameters so obtained are compared with those deduced isothermally. The results of the ABS method, at the lowest heating rate (q = 1.2 K min— '), agree well with the ...