نتایج جستجو برای: thermolabile
تعداد نتایج: 747 فیلتر نتایج به سال:
DNA synthesis in vitro in Brij-treated Saccharomyces cerevisiae requires the product of the CDC8 gene (Hereford, L. M. & Hartwell, L. H. (1971) Nature (London) New Biol. 234, 171-172). Extracts of wild-type A364a yeast restore DNA synthesis in Brij-treated cdc8, a mutant containing a thermolabile cdc8 gene product. This constitutes a complementation assay by which the cdc8 gene product can be m...
Murine sarcoma virus-associated protein kinases that bind to actin have been purified by affinity chromatography on actin coupled to Sepharose. Heat inactivation studies showed the presence of thermolabile enzyme activity in pseudotypes containing a temperature-sensitivity mutant of murine sarcoma virus (MSV) but not in two independent wild-type MSV pseudotypes. Studies with Sephadex G-75 colum...
A purification procedure for Neurospora crassa isocitrate lyase (ICL) is described. Thirteen mutants at the acu-3 locus produced no detectable ICL activity either on sucrose as sole carbon source or in the presence of acetate. These mutants also produced little or no protein able to neutralize anti-ICL antibodies. Selection of revertants, able to grow on acetate as sole carbon source, from one ...
A purification procedure for Neurospora crassa isocitrate lyase (ICL) is described. Thirteen mutants at the acu-3 locus produced no detectable ICL activity either on sucrose as sole carbon source or in the presence of acetate. These mutants also produced little or no protein able to neutralize anti-ICL antibodies. Selection of revertants, able to grow on acetate as sole carbon source, from one ...
The aim of this study was to identify hereditary and acquired risk-factors as they are related to the occurrence of stroke in children. We identified 21 children with stroke. A search of the Factor V Leiden mutation, the Factor II G20210A variant, and the thermolabile variant of methylenetetrahydrofolate reductase was performed in patients and in a control group (n = 115). We identified risk fa...
Tetraploid strains of Saccharomyces cerevisiae carrying different dosages of the CYR1+ gene have been constructed. Adenylate cyclase activity observed in these tetraploid strains was proportional to the dosage of the active CYR1+ gene. Of the 57 mutants requiring adenosine 3',5'-monophosphate for growth at 35 degrees C, two allelic temperature-sensitive cyr1 mutants produced thermolabile adenyl...
BACKGROUND Hyperhomocysteinemia, an independent and graded risk factor for coronary artery disease (CAD), may result from both environmental and hereditary factors. Methylenetetrahydrofolate reductase (MTHFR) catalyzes the conversion of methylenetetrahydrofolate to methyltetrahydrofolate, the methyl donor in the remethylation of homocysteine to methionine. A 677C-->T mutation in the MTHFR gene ...
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