T development of a systematic design process for synthetic gene regulatory networks is an intriguing prospect that could improve both our understanding of regulatory motifs and our techniques for constructing novel applications. Such a design process would entail analysis tools capable of reliably predicting the in vivo behavior resulting from a given regulatory architecture. As with the proces...

M ultivesicular bodies (MVBs) are specialized endosomes that promote the degradation of membrane proteins by delivering them to lysosomes. This process is regulated by a series of endosomal sorting complexes required for transport (ESCRTs) (1). The ESCRT-0,-I, and-II complexes sequester ubiquitinated membrane proteins and recruit the ESCRT-III complex to drive the invagination and release of ca...

Many real-world analytics problems involve two significant challenges: prediction and optimization. Due to the typically complex nature of each challenge, the standard paradigm is to predict, then optimize. By and large, machine learning tools are intended to minimize prediction error and do not account for how the predictions will be used in a downstream optimization problem. In contrast, we p...

CA) reveal that DNA is synthesized when it is least likely to be hit with oxidative damage. Damaging oxidizing agents are produced by respiration. In yeast, respiration is periodic—it alternates in ‫ف‬ 40-min cycles with a stage of non-respiration called the reductive phase. This oscillation was detected decades ago, but it was never thought to be connected to cell cycle progression. Now, Kleve...

DEVELOPING a softwareintensive system is like raising a child. As Will PleasantRyan observed, both babies and code have smells, you get used to changing requirements, you have to take the long view, you can’t do it alone, and eventually you have to let go.1 I would add to Will’s most excellent simile that, although you might think you know everything up front, you certainly don’t, and therefore...

"Slime" played a brief and spectacular role in the 19th century founded by the theory of primordial slime by Ernst Haeckel. However, that substance was never found and eventually abandoned. Further scientific attention slowly began in the 1930s referring to slime as a microbial product and then was inspired by "How bacteria stick" by Costerton et al. in 1978, and the matrix material was conside...

Fluoroquinolones were discovered in the 1960s as a derivative of the antimalarial drug chloroquine. Over the past 40 years, many fluoroquinolones have been developed for use in human and veterinary medicine. As with all classes of antibiotics, resistance to fluoroquinolones is a serious concern, and multiple avenues for resistance are being investigated. Resistance-associated point mutations in...

The themes presented in this Presidential address are introduced through masterpieces of medicine in art from the very earliest depictions to the modern day. The representations of medicine in art provide a commentary on the place of medicine in society and how it was viewed by society. Some artists used their work both to highlight important health issues of the time and as a " propaganda " to...