Superoxide dismutase (SOD) is an antioxidant enzyme that acts to degrade superoxide, a major causitive factor for oxidative stress associated with cancer, cardiovascular disease, and various other ailments. Here, to assess an association between antioxidants and lifestyle factors related to cancer risk, we analyzed serum SOD activity among the subjects within a large-scale cohort study in Japan...

Background: Withania somnifera L. Dunal, (Ashwagandha) belongs to the family Solanaceae and is known as ‘Indian ginseng’ in the traditional system of medicine. Objectives: In the present investigation, comparative analysis was carried out between two different ecotypes of W. somnifera related to total soluble protein and antioxidant enzyme activity [catalase (CAT), s...

Alpha amanitin and phalloidin are powerful hepatotoxins that belong to the main classes of toxins namely, amatoxins and phallotoxins, isolated from the deadly poisonous mushroom Amanita phalloides. It is well known that disruptions of antioxidant enzyme defense that include superoxide dismutase (SOD), catalase, glutathione peroxidase lead to generation of reactive oxygen species, followed by in...

Cyanobacteria are autotrophic prokaryotes with excellent antioxidative system that scavenges reactive oxygen species produced during photosynthesis. Superoxide dismutase (SOD) represent the primary defence against oxygen damage. The antioxidative activity of SOD enzymes was determined in cultures of the bloom forming cyanobacteria Anabaena variabilis and Synechococcus elongates. Experimental re...

background: the aim of this study was to compare superoxide dismutase (sod) activity in fasciola hepatica and fasciola gigantica parasites. materials: f. gigantica and f. hepatica helminths were collected from abattoir and cultured in buffer media for 4 h at 37 °c. excretory-secretory (es) products were collected, centrifuged and stored at - 20 ◦ c. e-s protein concentration was measured by bra...

Distribution profiles of superoxide dismutase isoenzymes in various tissues of rabbits with the Vx-2 carcinoma in the maxillary sinus were compared with those of control rabbits. Copper- and zinc-containing superoxide dismutase (Cu,Zn-SOD) activity in the liver of rabbits decreased significantly 3 weeks after transplantation. Manganese-containing superoxide dismutase (Mn-SOD) activity did not d...

A glyoxysomal copper,zinc-containing superoxide dismutase (EC 1.15.1.1) was purified to homogeneity, for the first time, from watermelon cotyledons (Citrullus vulgaris Schrad.). The stepwise purification procedure consisted of acetone precipitation, batch anion-exchange chromatography, anion-exchange Fast Protein Liquid Chromatography and gel-filtration column chromatography. Pure copper,zinc-s...

Superoxide dismutase (SOD) is an enzyme facilitating the removal of superoxide anions from living organisms. This study focused on SOD from the silkworm, Bombyx mori (bmSOD). cDNA encoding bmSOD was amplified by reverse transcriptase-polymerase chain reaction. The deduced amino acid sequence of bmSOD indicated that the residues forming the Cu/Zn binding site are conserved and that the sequence ...

PURPOSE The authors determined the levels of the secreted, interstitially located extracellular superoxide dismutase (EC-SOD), the cytosolic copper- and zinc-containing SOD (CuZn-SOD), and the mitochondrial manganese-containing SOD (Mn-SOD) in tears, cornea, sclera, aqueous humor, lens, vitreous body, and retina of the human eye. METHODS SOD enzymatic activity was determined by the direct spe...