Microfocusing X-rays direct high-density photons on crystal samples and can enhance the diffraction limit quality of collected data. However, these intense cause radiation damage to sample, which often results in undesirable structural information. Accordingly, a data collection strategy that minimizes is critical obtaining accurate In this study, single-point was investigated at two different ...

The accuracy of X-ray diffraction data depends on the properties of the crystalline sample and on the performance of the data-collection facility (synchrotron beamline elements, goniostat, detector etc.). However, it is difficult to evaluate the level of performance of the experimental setup from the quality of data sets collected in rotation mode, as various crystal properties such as mosaicit...

Phloem exudate collected from hybrid poplar (Populus trichocarpa x Populus deltoides) was estimated to have more than 100 proteins, of which 48 were identified using LC-MS/MS. Comparative two-dimensional gel electrophoresis demonstrated that two phloem exudate proteins were significantly (P<0.05) upregulated 24 h after leaf wounding. These were identified as pop3/SP1 and a thaumatin-like protei...

Plants are recurrently exposed to myriads of biotic and abiotic stresses leading several biochemical physiological variations that cause severe impacts on plant growth survival. To overcome these challenges, plants activate two primary defense mechanisms, such as structural response (cell wall strengthening waxy epidermal cuticle development) metabolic changes, including the synthesis anti-micr...